Alexa Fluor™ 488 Streptavidin, 5 nm colloidal gold conjugate - FAQs

I want to label F-actin with phalloidin and image with transmission electron microscopy. What is the best way to go about it?

One way is to use a biotin-phalloidin conjugate, then label that with a colloidal gold conjugate of streptavidin. You will need to block endogenous biotin first. Another method is to label with fluorescein phalloidin, then with an anti-fluorescein antibody conjugated with colloidal gold.

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