An Introductory Guide to Protease Sensitive Linker Design Using Matrix Metalloproteinase 13 as an Example.
AuthorsHamm P,Meinel L,Driessen MD
JournalACS biomaterials science & engineering
PubMed ID38813796
Proteases play a crucial role, not only in physiological, but also in pathological processes, such as cancer, inflammation, arthritis, Alzheimer's, and infections, to name but a few. Their ability to cleave peptides can be harnessed for a broad range of biotechnological purposes. To do this efficiently, it is essential to ... More
Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions.
Nuclease-inactivated CRISPR/Cas-based (dCas-based) systems have emerged as powerful technologies to synthetically reshape the human epigenome and gene expression. Despite the increasing adoption of these platforms, their relative potencies and mechanistic differences are incompletely characterized, particularly at human enhancer-promoter pairs. Here, we systematically compared the most widely adopted dCas9-based transcriptional activators, ... More
Not just fat: investigating the proteome of cetacean blubber tissue.
AuthorsKershaw JL,Botting CH,Brownlow A,Hall AJ
JournalConservation physiology
PubMed ID29479430
Mammalian adipose tissue is increasingly being recognized as an endocrine organ involved in the regulation of a number of metabolic processes and pathways. It responds to signals from different hormone systems and the central nervous system, and expresses a variety of protein factors with important paracrine and endocrine functions. This ... More