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Alexa Fluor™ 488 Carboxylic Acid, tris(triethylammonium) salt, 5 mg - FAQs

Alexa Fluor™ 488 Carboxylic Acid, tris(triethylammonium) salt, 5 mg - FAQs

查看更多产品信息 Alexa Fluor™ Carboxylic Acid, tris(triethylammonium) salts - FAQs (A33079, A33078, A33080, A33082, A33081, A33084, A33083, A33085, A33077, A33076)

7 个常见问题解答

注射之后多久可以对我的小鼠进行成像检测？

成像时间进程随注入试剂的性质而变化。血管示踪剂是在注射血管后立即显示，可在数小时后成像。偶联的全IgG抗体注射后的几个小时内到达目标位置，可成像数天。

小鼠体内未结合的染料会造成哪些后果？

染料会经由膀胱排出。膀胱信号可在染料静脉注射3分钟内检测到。

What dye can I use that is non-reactive and can show an injection site?

The non-reactive Alexa Fluor and Alexa Fluor hydrazide derivatives may be used for injection site visualization. Other options include the fluorescent polystyrene microspheres, FluoSpheres, and dye-conjugated dextrans. The hydrazide derivatives and 'fixable' dextrans are retained by cross-linking using an aldehyde-based fixative.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which optical filters are compatible with my Alexa Fluor free acid dye?

Please consult the Fluorescence SpectraViewer to find the optimal optical filters to use with your Alexa Fluor free acid dye (Cat. Nos. A33076, A33077, A33078, A33079, A33080, A33081, A33082, A33083, A33084, A33085).

For more information on how to use the Fluorescence SpectraViewer tool, please refer to the Guide for Using the Fluorescence SpectraViewer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I reconstitute and store my Alexa Fluor free acid dye?

Alexa Fluor free acid compounds (Cat. Nos. A33076, A33077, A33078, A33079, A33080, A33081, A33082, A33083, A33084, A33085) should be stored at -20 degrees C and protected from light until it is reconstituted.

To reconstitute, dissolve the Alexa Fluor free acid compound in aqueous buffer (e.g., PBS), or high-quality, anhydrous dimethylsulfoxide (DMSO) or dimethylformamide (DMF) at a final concentration of 1–10 mg/mL. Once reconstituted, this solution is stable for 1 year, when stored at 2-8 degrees C and protected from light.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long after injection should I start imaging my mouse?

The imaging time course varies with the nature of the injected agent. Vascular tracers are visible in the blood vessels immediately after injection and may be imaged for several hours. Conjugated whole IgG antibodies reach their targets within a few hours of injection and may be imaged for several days.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What happens to unconjugated dye in the mouse after small animal in vivo imaging?

The dye will be eliminated via the bladder. The bladder signal is detectable within ~3 minutes of IV injection of the dye and clearance with ~30 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.