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CultureOne™ Supplement (100X) - FAQs

CultureOne™ Supplement (100X) - FAQs

查看更多产品信息 CultureOne™ Supplement (100X) - FAQs (A3320201)

6 个常见问题解答

What are the storage conditions for CultureOne Supplement? Can I store it at 4 degrees C and for how long? Can I aliquot unused product and freeze it at -20 degrees C?

We recommend that you thaw the CultureOne Supplement and then aliquot it out for working stocks and re-freeze the aliquots. We have found that the product can undergo up to 3 freeze-thaws without loss of activity. CultureOne-supplemented media is good for 2 weeks stored at 4 degrees C in the dark.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am using the B-27 Plus Neuronal Culture System. What do I do if my neuronal cells are not attaching or are clumped?

In primary neuronal cultures, this is typically a function of uneven coating or if neurons are plated at too high of a density. In NSC differentiation experiments, this could be due to overproliferation of neural progenitor cells. Differentiating with the B-27 Plus Neuronal Culture System and CultureOne Supplement can eliminate more than 75% of neural progenitor cells and the clumps they form. Please see the CultureOne Supplement User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016204_CultureOneSupplement_UG.pdf) for more information.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I am planning to use the B-27 Plus Neuronal Culture System. How can I control the number of glial cells in my neuronal culture?

Add CultureOne Supplement at day 0 with the B-27 Plus Neuronal Culture System to fully suppress both astrocytes and oligodendrocytes with no detrimental effect on neurons. Delaying the addition of CultureOne Supplement to later time points results in increasing levels of astrocytes. Refer to the CultureOne Supplement User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016204_CultureOneSupplement_UG.pdf) for more information.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What specific types of neurons will CultureOne Supplement work with?

CultureOne Supplement accelerates the maturation of all types of differentiating neurons.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw CultureOne Supplement and re-freeze it?

CultureOne Supplement can be frozen and thawed up to 3 times without affecting the product performance.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to continue to add CultureOne Supplement as long as I continue to maintain the neuronal culture after 2 weeks?

It is better to treat differentiating neurons continuously with CultureOne Supplement. You can withdraw CultureOne Supplement after 2 weeks of differentiation; however, withdrawal of CultureOne Supplement may increase the chances of clumps reforming in the culture.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.