• 450 mL 基础培养基,避光储存于 2–8°C 条件下 • 50 mL 补充剂,避光储存于 -5 至 -20°C 条件下
常见问题解答 (FAQ)
What is the glucose content of the StemFlex Medium (Cat. No. A3349401)?
The StemFlex Medium (Cat. No. A3349401) includes 3.151 g/L (~17.5 mM) of D-glucose (dextrose) in the Basal Medium. The StemFlex Medium Supplement does not include glucose.
What media do you recommend for standard single-cell cloning of iPSCs?
We recommend StemFlex Medium (Cat. No. A3349401) or Essential 8 (Cat. No. A1517001) for standard single-cell iPSC cloning in the presence of RevitaCell or ROCK inhibitor. StemScale PSC Suspension Medium (Cat. No. A4965001) is not recommended for iPSC cloning.
What is the difference between StemFlex Medium and Essential 8 Flex Medium?
Essential 8 Flex Medium is a serum-free, xeno-free medium that contains proprietary material to extend the activity of the key heat-sensitive component, FGF2. Therefore, it supports the culture and expansion of feeder-free pluripotent stem cells (PSCs) without the need for daily or weekend feeding. Note: We also offer Essential 8 Medium that does not allow skipping of PSC feeding during the weekend.
StemFlex Medium also supports the expansion of feeder-free PSCs with a flexible feeding schedule (including weekend-free feeding) and also the ability to choose the matrix and passaging reagent that best suits specific applications.
Has StemFlex Medium been evaluated for growth of mouse ESCs?
StemFlex Medium contains basic FGF which has been shown to result in differentiation of mouse embryonic stem cells (mESCs) to mouse-derived epiblast stem cells (mEpiSCs). Also, mESCs demonstrate dependence on leukemia inhibitor factor (LIF). Therefore, StemFlex Medium is not recommended for culture of mESCs.