StemFlex™ 培养基
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StemFlex™ 培养基
引用和文献 (10)
Gibco™

StemFlex™ 培养基

Gibco™ StemFlex™ 培养基支持无滋养层多能干细胞 (PSC) 的稳定扩增，经过优化可支持单细胞传代、基因编辑和重编程等新型应用。StemFlex 培养液独特的配方通过灵活的滋养时间表（包括周末无需滋养选项）提供了便利，并且支持选择适合特定应用的基质和传代试剂了解更多信息
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货号数量
A3349401500 mL
货号 A3349401
价格（CNY)
2,411.00
飞享价
Ends: 27-Dec-2025
4,082.00
共减 1,671.00 (41%)
Each
添加至购物车
数量:
500 mL
价格（CNY)
2,411.00
飞享价
Ends: 27-Dec-2025
4,082.00
共减 1,671.00 (41%)
Each
添加至购物车
Gibco™ StemFlex™ 培养基支持无滋养层多能干细胞 (PSC) 的稳定扩增，经过优化可支持单细胞传代、基因编辑和重编程等新型应用。StemFlex 培养液独特的配方通过灵活的滋养时间表（包括周末无需滋养选项）提供了便利，并且支持选择适合特定应用的基质和传代试剂。StemFlex 培养基可实现长期的无滋养层 PSC 培养而没有任何核型异常，并保持了细胞分化为全部三个胚层的能力。

卓越的结果
StemFlex 培养基配方可为当今较为复杂的给 PSC 培养带来压力的应用提供卓越的结果，如重编程、单细胞传代和基因编辑。与领先的竞争产品 (mTeSR1) 相比，StemFlex 培养基可提供出色的细胞存活率，帮助在较为相关的细胞类型中取得更大的成功。

灵活性
培养基可为 PSC 培养条件提供前所未有的 3 向使用灵活性：能够选择较优的滋养时间表，包括一个无需周末滋养的选项；根据工作流程需求选择基质；以及用于选择所需细胞解离水平的传代试剂的选项。

优异的价值和性能
StemFlex 培养基以一种便利的两组分试剂盒（450 mL 基础培养基和 50 mL 补充剂）形式提供，在与 Geltrex™ 无 LDEV、hESC 合格的低生长因子基底膜基质配合使用时，可为人 PSC 无饲养层培养提供一种经济高效的稳健系统。StemFlex 培养基能够实现无需周末滋养的 PSC 培养，为当今市场上稳健的无滋养层 PSC 培养基提供了无与伦比的价值。

仅供科研使用。不可用于诊断程序。
规格
细胞系Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
细胞类型胚胎干细胞、诱导多能干细胞
最大浓度StemFlex Basal Medium (1X); StemFlex Supplement (10X)
生产质量符合医疗器械 cGMP（21 CFR 第 820 部分）和 ISO 13485
产品线StemFlex
产品类型干细胞培养基
数量500 mL
有效期12 个月
运输条件补充剂用干冰运输，基础培养基可在室温条件下运输
分类非动物源性
培养类型无饲养层 PSC 培养, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
形式Liquid
血清水平Serum-free
无菌Sterile-filtered
灭菌方法Sterile-filtered
加有添加剂酚红
Unit SizeEach
内容与储存
• 450 mL 基础培养基，避光储存于 2–8°C 条件下
• 50 mL 补充剂，避光储存于 -5 至 -20°C 条件下

常见问题解答 (FAQ)

What is the glucose content of the StemFlex Medium (Cat. No. A3349401)?

The StemFlex Medium (Cat. No. A3349401) includes 3.151 g/L (~17.5 mM) of D-glucose (dextrose) in the Basal Medium. The StemFlex Medium Supplement does not include glucose.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What media do you recommend for standard single-cell cloning of iPSCs?

We recommend StemFlex Medium (Cat. No. A3349401) or Essential 8 (Cat. No. A1517001) for standard single-cell iPSC cloning in the presence of RevitaCell or ROCK inhibitor. StemScale PSC Suspension Medium (Cat. No. A4965001) is not recommended for iPSC cloning.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does StemFlex Medium contain pen/strep antibiotic?

StemFlex Medium does not contain pen/strep antibiotic.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between StemFlex Medium and Essential 8 Flex Medium?

Essential 8 Flex Medium is a serum-free, xeno-free medium that contains proprietary material to extend the activity of the key heat-sensitive component, FGF2. Therefore, it supports the culture and expansion of feeder-free pluripotent stem cells (PSCs) without the need for daily or weekend feeding. Note: We also offer Essential 8 Medium that does not allow skipping of PSC feeding during the weekend.

StemFlex Medium also supports the expansion of feeder-free PSCs with a flexible feeding schedule (including weekend-free feeding) and also the ability to choose the matrix and passaging reagent that best suits specific applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Has StemFlex Medium been evaluated for growth of mouse ESCs?

StemFlex Medium contains basic FGF which has been shown to result in differentiation of mouse embryonic stem cells (mESCs) to mouse-derived epiblast stem cells (mEpiSCs). Also, mESCs demonstrate dependence on leukemia inhibitor factor (LIF). Therefore, StemFlex Medium is not recommended for culture of mESCs.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all StemFlex™ 培养基 FAQs

引用和文献 (10)

引用和文献
Abstract
Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media.
Authors:Daniszewski M, Nguyen Q, Chy HS, Singh V, Crombie DE, Kulkarni T, Liang HH, Sivakumaran P, Lidgerwood GE, Hernández D, Conquest A, Rooney LA, Chevalier S, Andersen SB, Senabouth A, Vickers JC, Mackey DA, Craig JE, Laslett AL, Hewitt AW, Powell JE, Pébay A
Journal:iScience
PubMed ID:30267684
'We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells ... More
Improving single-cell cloning workflow for gene editing in human pluripotent stem cells.
Authors:Chen YH, Pruett-Miller SM
Journal:Stem Cell Res
PubMed ID:30099335
'The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens. Unfortunately, the procedures for genome editing of hPSCs often subject cells to harsh conditions that compromise viability: a major problem that is compounded by ... More
Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing.
Authors:Weisheit I, Kroeger JA, Malik R, Klimmt J, Crusius D, Dannert A, Dichgans M, Paquet D
Journal:Cell Rep
PubMed ID:32460021
'CRISPR genome editing is a promising tool for translational research but can cause undesired editing outcomes, both on target at the edited locus and off target at other genomic loci. Here, we investigate the occurrence of deleterious on-target effects (OnTEs) in human stem cells after insertion of disease-related mutations by ... More
Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells.
Authors:Chen SW, Himeno M, Koui Y, Sugiyama M, Nishitsuji H, Mizokami M, Shimotohno K, Miyajima A, Kido T
Journal:Sci Rep
PubMed ID:32873852
'Hepatocytes derived from human iPSCs are useful to study hepatitis B virus (HBV) infection, however infection efficiency is rather poor. In order to improve the efficiency of HBV infection to iPSC-derived hepatocytes, we set a co-culture of hepatocytes with liver non-parenchymal cells and found that liver sinusoidal endothelial cells (LSECs) ... More
A Simple Procedure for Creating Scalable Phenotypic Screening Assays in Human Neurons.
Authors:Sridharan B, Hubbs C, Llamosas N, Kilinc M, Singhera FU, Willems E, Piper DR, Scampavia L, Rumbaugh G, Spicer TP
Journal:Sci Rep
PubMed ID:31227747
'Neurons created from human induced pluripotent stem cells (hiPSCs) provide the capability of identifying biological mechanisms that underlie brain disorders. IPSC-derived human neurons, or iNs, hold promise for advancing precision medicine through drug screening, though it remains unclear to what extent iNs can support early-stage drug discovery efforts in industrial-scale ... More
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