Cardioprotective proteins upregulated in the liver in response to experimental myocardial ischemia.
AuthorsLiu SQ, Tefft BJ, Roberts DT, Zhang LQ, Ren Y, Li YC, Huang Y, Zhang D, Phillips HR, Wu YH,
JournalAm J Physiol Heart Circ Physiol
PubMed ID23064833
'Myocardial ischemia (MI) activates innate cardioprotective mechanisms, enhancing cardiomyocyte tolerance to ischemia. Here, we report a MI-activated liver-dependent mechanism for myocardial protection. In response to MI in the mouse, hepatocytes exhibited 6- to 19-fold upregulation of genes encoding secretory proteins, including a-1-acid glycoprotein (AGP)2, bone morphogenetic protein-binding endothelial regulator (BMPER), ... More
High-throughput investigation of osteoblast response to polymer crystallinity: influence of nanometer-scale roughness on proliferation.
AuthorsWashburn NR, Yamada KM, Simon CG, Kennedy SB, Amis EJ
JournalBiomaterials
PubMed ID14643595
'A high-throughput method for analyzing cellular response to crystallinity in a polymer material is presented. Variations in crystallinity lead to changes in surface roughness on nanometer length scales, and it is shown that cells are exquisitely sensitive to these changes. Gradients of polymer crystallinity were fabricated on films of poly(L-lactic ... More
AuthorsBeazely MA, Lim A, Li H, Trepanier C, Chen X, Sidhu B, Macdonald JF,
JournalJ Biol Chem
PubMed ID19106110
'Platelet-derived growth factor (PDGF) beta receptor activation inhibits N-methyl-d-aspartate (NMDA)-evoked currents in hippocampal and cortical neurons via the activation of phospholipase Cgamma, PKC, the release of intracellular calcium, and a rearrangement of the actin cytoskeleton. In the hippocampus, the majority of NMDA receptors are heteromeric; most are composed of 2 ... More
A digital microfluidic approach to heterogeneous immunoassays.
AuthorsMiller EM, Ng AH, Uddayasankar U, Wheeler AR,
JournalAnal Bioanal Chem
PubMed ID21057776
A digital microfluidic (DMF) device was applied to a heterogeneous sandwich immunoassay. The digital approach to microfluidics manipulates samples and reagents in the form of discrete droplets, as opposed to the streams of fluid used in microchannels. Since droplets are manipulated on relatively generic 2-D arrays of electrodes, DMF devices ... More