Red blood cell band 3. Lysine 539 and lysine 851 react with the same H2DIDS (4,4'-diisothiocyanodihydrostilbene-2,2'-disulfonic acid) molecule.
AuthorsOkubo K, Kang D, Hamasaki N, Jennings ML
JournalJ Biol Chem
PubMed ID8294441
The band 3 protein of the red blood cell membrane catalyzes anion exchange that is inhibited by the stilbenedisulfonate derivative H2DIDS (4,4'-diisothiocyanodihydrostilbene-2,2'-disulfonic acid). There is one H2DIDS binding site per 95,000-Da band 3 polypeptide. The single bound H2DIDS molecule can react covalently with 2 different lysine residues. The 2 lysines ... More
The mechanisms of inhibition of anion exchange in human erythrocytes by 1-ethyl-3-[3-(trimethylammonio)propyl]carbodiimide.
AuthorsWerner PK, Reithmeier RA
JournalBiochim Biophys Acta
PubMed ID2454665
'Treatment of human erythrocytes with the membrane-impermeant carbodiimide 1-ethyl-3-[3-(trimethylammonio)propyl]carbodiimide (ETC) in citrate-buffered sucrose leads to irreversible inhibition of phosphate-chloride exchange. The level of transport inhibition produced was dependent on the concentration of citrate present during treatment, with a maximum of approx. 60% inhibition. [14C]Citric acid was incorporated into Band 3 ... More
Regulation of intracellular pH in the perfused heart by external HCO3- and Na(+)-H+ exchange.
AuthorsGrace AA, Kirschenlohr HL, Metcalfe JC, Smith GA, Weissberg PL, Cragoe EJ, Vandenberg JI
JournalAm J Physiol
PubMed ID8393626
'Both Na(+)-dependent HCO3- influx and the Na(+)-H+ antiport have been shown to contribute to recovery from intracellular acidosis in avian and mammalian cardiac tissue. We have investigated the participation of these mechanisms in the recovery of intracellular pH (pHi) after an acid load in the Langendorff-perfused ferret heart. pHi was ... More
Analysis of ion transport perturbations caused by hu MDR 1 protein overexpression.
AuthorsHoffman MM, Roepe PD
JournalBiochemistry
PubMed ID9287158
'In previous work [Luz et al. (1994) Biochemistry 33, 7239-7249; Roepe et al. (1994) Biochemistry 33, 11008-11015] we measured changes in Cl- and HCO3--dependent pHi regulation for LR73 Chinese hamster ovary fibroblasts overexpressing mu MDR 1 protein. However, only one clonal cell line overexpressing the protein but not previously exposed ... More
Transport of organic anions through the erythrocyte membrane as K+-valinomycin complexes.
AuthorsMarinetti GV, Skarin A, Whitman P
JournalJ Membr Biol
PubMed ID660643
'K+, Rb+, or Cs+ complexes of valinomycin form ion pair complexes with picric acid and trinitrobenzenesulfonate (TNBS). The formation of a picrate-K+-valinomycin complex is supported by spectral evidence. These complexes have zero net charge and readily permeate the intact erythrocyte membrane. The K+-valinomycin complex has been used to convert the ... More
Uptake of hexavalent chromium by bovine erythrocytes and its interaction with cytoplasmic components; the role of glutathione.
AuthorsKitagawa S, Seki H, Kametani F, Sakurai H
JournalChem Biol Interact
PubMed ID6282472
'Hexavalent chromium (Cr(VI)) anion gradually penetrated into bovine erythrocytes and bound with cytoplasmic components. Its penetration was strongly inhibited by the NH2-reactive agent, 4-acetamido-4''-isothiocyano-stilbene-2,2''-disulfonic acid (SITS) and the SH-reactive agent, N-ethylmaleimide (NEM). Gel filtration showed that the intracellular component that bound to chromium was hemoglobin. The binding affinity of Cr(VI) ... More
Band 3 Memphis variant II. Altered stilbene disulfonate binding and the Diego (Dia) blood group antigen are associated with the human erythrocyte band 3 mutation Pro854-->Leu.
AuthorsBruce LJ, Anstee DJ, Spring FA, Tanner MJ
JournalJ Biol Chem
PubMed ID8206915
'Band 3 Memphis is a commonly occurring polymorphic form of the human red cell anion transporter (band 3, AE1). Band 3 Memphis migrates more slowly on an SDS-polyacrylamide gel than normal band 3 and results from a point mutation Lys56-->Glu. Two types of band 3 Memphis, variants I and II, ... More
Rat hepatocytes exhibit basolateral Na+/HCO3- cotransport.
AuthorsRenner EL, Lake JR, Scharschmidt BF, Zimmerli B, Meier PJ
JournalJ Clin Invest
PubMed ID2539394
'Primary cultures and plasma membrane vesicles were used to characterize Na+ and HCO3- transport by rat hepatocytes. Na+ uptake into hepatocytes was stimulated approximately 10-fold by 25 mM extracellular HCO3-.HCO3--stimulated Na+ uptake was saturable, abolished by 4-acetamido-4''-isothiocyano-2,2''-disulfonic acid stilbene (SITS), and unaffected by amiloride or Cl- removal. Neither propionate nor ... More
Reversible and irreversible inhibition, by stilbenedisulphonates, of lactate transport into rat erythrocytes. Identification of some new high-affinity inhibitors.
AuthorsPoole RC, Halestrap AP
JournalBiochem J
PubMed ID2025218
'1. Inhibition of L-lactate transport into rat erythrocytes by stilbenedisulphonates was studied under conditions which allowed the contribution of reversible and irreversible inhibition to be assessed. 2. At low temperatures (7 degrees C), 4,4''-di-isothiocyanostilbene-2,2''-disulphonate (DIDS) and other stilbenedisulphonates were found to inhibit lactate transport instantaneously, in a manner which was ... More
Sulfate transport in rabbit proximal convoluted tubules: presence of anion exchange.
AuthorsBrazy PC, Dennis VW
JournalAm J Physiol
PubMed ID7025658
'These studies describe the characteristics of sulfate transport in proximal convoluted tubules from rabbit kidney. We measured absorptive and secretory fluxes of sulfate in isolated tubular segments perfused and bathed with fluids containing sulfate concentrations of 0.2-10 mM. At 2.0 mM, the sulfate flux in the absorptive direction averaged 4.76 ... More
Distribution of chloride and potassium in cellular and luminal compartments of control and drug-treated turtle thyroid.
AuthorsChow SY, Woodbury DM, Yen-Chow YC
JournalJ Physiol
PubMed ID6310090
'Chloride and potassium activities inside the lumen and the transepithelial potentials of control and drug-treated turtle thyroid follicles were determined simultaneously by ion-selective and conventional 3 M-KCl micro-electrodes, respectively. Water and electrolyte contents of these thyroid tissues were determined chemically after each experiment. Cellular and luminal concentrations of Cl- and ... More
A novel UDP-Glc-specific glucosyltransferase catalyzing the biosynthesis of 6-O-glucosides of bile acids in human liver microsomes.
AuthorsRadominska A, Little J, Pyrek JS, Drake RR, Igari Y, Fournel-Gigleux S, Magdalou J, Burchell B, Elbein AD, Siest G
JournalJ Biol Chem
PubMed ID8325887
'Two active site-directed photoaffinity analogs, 5-[beta-32P]azido-UDP-glucuronic acid and 5-[beta-32P]azido-UDP-glucose, were used for the characterization of UDP-sugar-utilizing enzymes in human liver microsomes. Both compounds were recognized by human microsomal proteins: major photolabeled bands of 50-56 kDa were detected. Both photoincorporations were competitively decreased by increasing concentrations of either UDP-Glc or UDP-GlcUA, ... More
Similarity of lysosomal H+-ATPase to mitochondrial F0F1-ATPase in sensitivity to anions and drugs as revealed by solubilization and reconstitution.
AuthorsMoriyama Y, Takano T, Ohkuma S
JournalBiochim Biophys Acta
PubMed ID2867782
'Lysosomal H+-translocating ATPase (H+-ATPase) was solubilized with lysophosphatidylcholine and reconstituted into liposomes (Moriyama, Y., Takano, T. and Ohkuma, S. (1984) J. Biochem. (Tokyo) 96, 927-930). In this study, the sensitivities of membrane-bound, solubilized and liposome-incorporated ATPase to various anions and drugs were measured in comparison with those of similar forms ... More
pH sensitivity of the Na+:HCO3- cotransporter in basolateral membrane vesicles isolated from rabbit kidney cortex.
AuthorsSoleimani M, Hattabaugh YJ, Bizal GL
JournalJ Biol Chem
PubMed ID1526975
'HCO3- exit across the basolateral membrane of the kidney proximal tubule cell is mediated via an electrogenic Na+:HCO3- cotransporter. We have studied the effect of pH on the activity of this cotransport system in basolateral membrane vesicles isolated from rabbit renal cortex. At constant internal pH 6.0, increasing the external ... More
H+ and HCO3- flux across apical surface of rat distal colon.
AuthorsFeldman GM, Stephenson RL
JournalAm J Physiol
PubMed ID2164783
'Colonic ion transport is postulated to occur via simultaneous operation of Na(+)-H+ exchange and Cl(-)-HCO3- exchange. Accordingly H+ and HCO3- should be transported simultaneously by the colon. To assess simultaneous H+ and HCO3- transport, net acid-base flux was measured in isolated segments of rat distal colon. When both tissue surfaces ... More
The effects of anion transport inhibitors on structural transitions in erythrocyte membranes.
AuthorsSnow JW, Brandts JF, Low PS
JournalBiochim Biophys Acta
PubMed ID708734
'Red blood cell membranes have been labeled with several covalent and noncovalent inhibitors of anion transport and their heat capacity profiles determined as a function of temperature. Covalent inhibitors include the amino reactive agents 4,4''-diisothiocyanostilbene-2,2''-disulfonic acid, 4-acetamido-4''-isothiocyanostilbene-2,2''-disulfonic acid, pyridoxal phosphate and 1-fluoro-2,4-dinitro benzene. The non-covalent inhibitors include several well known ... More
A new type of two-color fluorescence staining for cytology specimens.
AuthorsCornelisse CJ, Ploem JS
JournalJ Histochem Cytochem
PubMed ID56393
'A new two-color fluorescence staining technique for cervical cytology specimens is described. To permit application of this staining in automated cytology, techniques for specimen collection and cell preparation giving a sufficient number of well-separated cells on slides were used. The staining consists of a combination of a modified Feulgen-acriflavine procedure ... More
Cl- fluxes related to fluid secretion by the rat parotid: involvement of Cl(-)-HCO3- exchange.
AuthorsMelvin JE, Turner RJ
JournalAm J Physiol
PubMed ID1312791
'Muscarinic-induced 36Cl- and 86Rb+ (K+ substitute) fluxes were studied in rat parotid acini. Stimulation resulted in a rapid [half time (t1/2) less than 30 s] decrease in both Cl- and Rb+ content (approximately 50 and 30%, respectively) followed by a slower partial recovery (t1/2 approximately 3-4 min) to approximately 80% ... More
Transport of gamma-aminobutyrate and L-glutamate into synaptic vesicles. Effect of different inhibitors on the vesicular uptake of neurotransmitters and on the Mg2(+)-ATPase.
AuthorsFykse EM, Fonnum F
JournalBiochem J
PubMed ID1675566
'The uptakes of gamma-aminobutyrate (GABA) and L-glutamate into synaptic vesicles isolated from rat brain were compared with respect to the effects of 4-acetamido-4''-isothiocyanostilbene-2,2''- disulphonic acid (SITS), 4,4''-di-isothiocyanostilbene-2,2''-disulphonic acid (DIDS) and 5-nitro-2-(3-phenylpropylamino)benzoic acid (N144), agents known to block anion channels. The uptake of glutamate was inhibited by low micromolar concentrations of ... More
Cytosolic pH regulation in mouse macrophages. Characteristics of HCO3(-)-dependent mechanisms.
AuthorsTapper H, Sundler R
JournalBiochem J
PubMed ID1310007
'Mechanisms regulating cytosolic pH (pHi) in adherent resident mouse macrophages have been characterized by use of the pH-sensitive fluorescent probe 2'',7''-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). Na+/H+ exchange was activated after an acid load of the macrophage cytosol. However, when Na+/H+ exchange was the only pHi-regulatory mechanism operative, recovery did not proceed beyond a ... More
Interactions of inhibitors on anion transporter of human erythrocyte.
AuthorsFröhlich O, Gunn RB
JournalAm J Physiol
PubMed ID3826335
'Chloride tracer efflux was measured from intact human erythrocytes into media containing different chloride concentrations and different concentrations of the inhibitors 4,4''-dinitrostilbene-2-2''-disulfonate (DNDS), N-(4-azido-2-nitrophenyl)-2-aminoethylsulfonate (NAP-taurine), phloretin, and sulfate. The data were analyzed to test whether these inhibitors were mutually exclusive with each other or whether they could bind at the ... More
Selective neuronal uptake of 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS) and other related substituted stilbenes in vivo: a fluorescent whole cell staining technique.
AuthorsSchmued LC, Fallon JH
JournalBrain Res
PubMed ID2413962
'The uptake of fluorescent stilbene derivatives including 4-acetamido-4''-isothiocyanostilbene-2,2''-disulfonic acid (SITS) was shown to occur from the blood or cerebrospinal fluid in the adult albino rat. SITS was found to be internalized in select populations of neurons, especially pyramidal neurons in layers V and VI of neocortex, granule, hilar and pyramidal ... More
Effects of inhibitors on anion exchangers in rabbit renal brush border membrane vesicles.
AuthorsMcConnell KR, Aronson PS
JournalJ Biol Chem
PubMed ID8063783
'Cl-formate, Cl-oxalate, and SO4-CO3 exchange participate in Cl and organic anion transport across the brush border membrane of the rabbit proximal tubule. To determine the functional similarity of these transporters to each other and to band 3, we characterized, in isolated membrane vesicles, the inhibition of these transporters by compounds ... More
Urea and ethylene glycol-facilitated transport systems in the human red cell membrane. Saturation, competition, and asymmetry.
AuthorsMayrand RR, Levitt DG
JournalJ Gen Physiol
PubMed ID6842173
'The equilibrium exchange of [14C]urea and ethylene glycol was measured using a new type of fast flow system. Approximately equal volumes of saline and air were mixed to form a segmented fluid stream into which 14C-loaded red cells are injected. The stream flows through three filter chambers which allow sampling ... More
'The activation of metabotropic glutamate receptors (mGluRs) by 1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD) or ibotenate induced a rapid cell volume increase in primary cultures of type 1 astroglial cells from the cerebral cortex of a newborn rat. These relative volume changes and parallel Ca2+ transients in single cells were examined by microspectrofluorimetry ... More
Endotoxin-induced degranulation of the Limulus amebocyte.
AuthorsArmstrong PB, Rickles FR
JournalExp Cell Res
PubMed ID6286331
Halide- and gamma-aminobutyric acid-induced enhancement of diazepam receptors in rat brain. Reversal by disulfonic stilbene blockers of anion channels.
AuthorsCosta T, Russell L, Pert CB, Rodbard D
JournalMol Pharmacol
PubMed ID6276708
Activation of the Na(+)/H(+) antiporter, Na+/HCO3(-)/CO3(2-) cotransporter, or Cl(-)/HCO3(-) exchanger in spontaneous thymocyte apoptosis.
AuthorsTsao N, Lei HY
JournalJ Immunol
PubMed ID8757615
'This study was undertaken to define the role of ion transporters on the apoptosis of thymocytes. Culture conditions, such as the ionic strength of NaCl, Ca2+, the buffer system (HCO3-/CO2), and the pH, could influence the spontaneous apoptosis of thymocytes. Depletion of NaCl in the culture medium either delayed or ... More
Investigation on the interactions of peptides in the assembly of liposome and peptide by fluorescence.
AuthorsUemura A, Kimura S, Imanishi Y
JournalBiochim Biophys Acta
PubMed ID6830785
'The peptide-lipid and the peptide-peptide interactions of hydrophobic linear dipeptides containing tryptophan in liposome were investigated by fluorescence. The linear dipeptides were buried into the hydrophobic region of liposome to induce blue-shift of the fluorescence. With the addition of various anthracene derivatives to liposome, the energy transfer from tryptophan to ... More
Endogenous K+/H+ exchange activity in the Sf9 insect cell line.
AuthorsVachon V, Paradis MJ, Marsolais M, Schwartz JL, Laprade R
JournalBiochemistry
PubMed ID7578130
'The fluorescent pH indicator 2'',7''-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to investigate changes in the intracellular pH (pHi) of individual Sf9 cells in response to changes in the composition of the external medium. Under standard conditions, the resting pHi was 0.2-0.3 unit lower than the extracellular pH (6.5). The extracellular concentration of ... More
Electron spin resonance studies on the inorganic-anion-transport system of the human red blood cell. Binding of a disulfonatostilbene spin label (NDS-TEMPO) and inhibition of anion transport.
AuthorsSchnell KF, Elbe W, Käsbauer J, Kaufmann E
JournalBiochim Biophys Acta
PubMed ID6307363
'The disulfonatostilbene spin label, NDS-TEMPO, was synthesized (purity over 96%) and the binding of the spin label to human red-cell ghosts was studied. NDS-TEMPO is readily absorbed to the membrane surface. Both pretreatment of the ghosts with FDNB and DIDS and the presence of DNDS completely prevent the binding of ... More
Phosphate transport by capillaries of the blood-brain barrier.
AuthorsDallaire L, Béliveau R
JournalJ Biol Chem
PubMed ID1429584
'Capillaries were isolated from bovine brain cortex and used for phosphate transport studies. The influx of phosphate through capillary membranes was studied by incubation with [32Pi]phosphate followed by a rapid filtration technique. Phosphate uptake by brain capillaries was mediated by a saturable high-affinity system which is independent of the sodium ... More
The interaction of intracellular Mg2+ and pH on Cl- fluxes associated with intracellular pH regulation in barnacle muscle fibers.
AuthorsRussell JM, Brodwick MS
JournalJ Gen Physiol
PubMed ID3392519
'The intracellular dialysis technique was used to measure unidirectional Cl- fluxes and net acid extrusion by single muscle fibers from the giant barnacle. Decreasing pHi below normal levels of 7.35 stimulated both Cl- efflux and influx. These increases of Cl- fluxes were blocked by disulfonic acid stilbene derivatives such as ... More
A protein-tyrosine kinase-regulated, pH-dependent, carrier-mediated uptake system for folate in human normal colonic epithelial cell line NCM460.
AuthorsKumar CK, Moyer MP, Dudeja PK, Said HM
JournalJ Biol Chem
PubMed ID9045638
'A significant proportion of the bacterially synthesized folate in the large intestine exists in the form of folate monoglutamate. Recent studies in our laboratory using human colonic apical membrane vesicles have shown the existence of an efficient carrier-mediated system for folate uptake. Nothing, however, is known about the cellular regulation ... More
Anion transport across the erythrocyte membrane, in situ proteolysis of band 3 protein, and cross-linking of proteolytic fragments by 4,4'-diisothiocyano dihydrostilbene-2,2'-disulfonate.
AuthorsJennings ML, Passow H
JournalBiochim Biophys Acta
PubMed ID486455
'Extracellular chymotrypsin cleaves the 95 000 dalton protein that migrates in band 3 of SDS-polyacrylamide gel electropherograms of the erythrocyte membrane into fragments of 60 000 and 35 000 daltons, but not further. Minor components of band 3 that remain at the original 95 000 dalton location may be eluted ... More
Organization of the striatum: collateralization of its efferent axons.
AuthorsLoopuijt LD, van der Kooy D
JournalBrain Res
PubMed ID4063830
'The anatomical structure of the basal ganglia indicates that the input from the cerebral cortex is funnelled through the striatum to the globus pallidus and substantia nigra. This structure implies integration of the information as it is transferred through the basal ganglia. In order to investigate this integration, we studied ... More
Effects of SITS on urate transport by isolated, perfused snake renal tubules.
AuthorsMukherjee SK, Dantzler WH
JournalPflugers Arch
PubMed ID3982957
'Effects of an inhibitor of membrane anion-exchange transport processes, 4-acetamido-4''-isothiocyano-2,2''-disulfonic stilbene (SITS), on urate transport by isolated, perfused snake (Thamnophis spp.) proximal renal tubules were studied. SITS (10(-4) mol/l) in the luminal perfusate had absolutely no effect on net urate secretion (Jneturate) or on net fluid absorption (JV). This observation ... More
Identification, purification, and characterization of a stilbenedisulfonate binding glycoprotein from canine kidney brush border membranes. A candidate for a renal anion exchanger.
AuthorsPimplikar SW, Reithmeier RA
JournalJ Biol Chem
PubMed ID3346257
'Canine renal brush border membrane proteins that bind stilbenedisulfonate inhibitors of anion exchange were identified by affinity chromatography. A 130-kDa integral membrane glycoprotein from brush border membrane was shown to bind specifically to 4-acetamido-4''-isothiocyanostilbene-2,2''-disulfonate immobilized on Affi-Gel 102 resin. The bound protein could be eluted effectively with 1 mM 4-benzamido-4''-aminostilbene-2,2''-disulfonate ... More
Cl-/HCO-3 antiporter in red cell ghosts: a kinetic assessment with fluorescent probes.
AuthorsLondon RD, Lipkowitz MS, Abramson RG
JournalAm J Physiol
PubMed ID3034076
'The pH sensitive fluorescent probe acridine orange and membrane potential-sensitive fluorescent probe acridine orange and membrane potential-sensitive fluorescent probe 3,3''-dipropylthiadicarbocyanine iodide were used to evaluate the Cl-/HCO-3 antiporter and proton and potassium conductances, respectively, in human red blood cell ghosts. Acidic, chloride-loaded ghosts alkalinized rapidly in pH 8.5 chloride-free media. ... More
Influence of bafilomycin A1 on pHi responses in cultured rabbit nonpigmented ciliary epithelium.
AuthorsWu Q, Delamere NA
JournalAm J Physiol
PubMed ID9374657
'Aqueous humor secretion is in part linked to HCO3- transport by nonpigmented ciliary epithelium (NPE) cells. During this process, the cells must maintain stable cytoplasmic pH (pHi). Because a recent report suggests that NPE cells have a plasma membrane-localized vacuolar H(+)-ATPase, the present study was conducted to examine whether vacuolar ... More
Human placental sulphate transport: studies on chorionic trophoblast brush border membrane vesicles.
AuthorsBoyd CA, Shennan DB
JournalJ Physiol
PubMed ID3795086
'SO4(2-) transport by microvillous brush border membrane vesicles prepared from normal-term human placenta has been studied using an ion-exchange column assay. The uptake of this anion is time dependent and at 20 degrees C reaches a point of equilibrium by 60 min. 4,4''-Diisothiocyanostilbene-2,2''-disulphonate (DIDS) and 4-acetamido-4''-isothiocyanostilbene-2-2''-disulphonate (SITS) (at 10(-4) M) ... More
A role for Na+/H+ exchange in pH regulation in Helix neurones.
AuthorsWilloughby D, Thomas RC, Schwiening CJ
JournalPflugers Arch
PubMed ID10591060
'We have used the pH-sensitive fluorescent dye 8-hydroxypyrene-1,3,6-trisulphonic acid (HPTS) to reexamine the mechanisms that extrude acid from voltage-clamped Helix aspersa neurones. Intracellular acid loads were imposed by three different methods: application of weak acid, depolarization and removal of extracellular sodium. In nominally CO2/HCO3-free Ringer the rate of recovery from ... More
Immunofluorescence studies with 4-acetamido-4'-isothiocyanato stilbene-2,2'-disulphonic acid (SITS).
AuthorsRothbarth PH, Tanke HJ, Mul NA, Ploem JS, Vliegenthart JF, Ballieux RE
JournalJ Immunol Methods
PubMed ID342607
'The application of the fluorochrome 4-acetamido-4''-isothiocyanato stilbene-2,2''-disulphonic acid (SITS) in immunofluorescence was studied. The optimal excitation wave length is 350 nm, and optimal fluorescence is obtained at 420 nm. After purification of the commercial compound, conjugation is performed in a strong buffer at pH 9.0-9.5. SITS conjugates were very satisfactory ... More
Low intracellular pH and chemical agents slow inactivation gating in sodium channels of muscle.
AuthorsNonner W, Spalding BC, Hille B
JournalNature
PubMed ID6767194
'Excitation of nerve or muscle requires an orderly opening and closing of molecular pores, the ionic channels, in the plasma membrane. During the action potential, Na channels are opened (activated) by the advancing wave of depolarisation, contributing a pulse of inward sodium current, and then are closed again (inactivated) by ... More
Variation between different samples of SITS with respect to axonal transport and toxicity.
AuthorsPayne JN, Lawes IN, Proctor GB, Horobin RW
JournalNeurosci Lett
PubMed ID6198609
'Retrograde axonal transport of the fluorescent compound SITS has been described as occurring only from axon terminals and not from axons of passage. Injection of 4 different commercially available samples of SITS into terminations of cerebellar pathways in the rat revealed that only one sample produced retrogradely labelled neurones. Chemical ... More
Characterization of yeast strains of the genera candida, Hansenula, Kluyveromyces, Pichia, Rhodotorula, and Saccharomyces by mixed-dye fluorometry.
AuthorsGeyer W, Brueggemann L, Flemming I, Nagel B
JournalInt J Syst Bacteriol
PubMed ID1854639
'An analytical method in which we used the selective adsorption of several fluorophores by yeast cells is described. The suitability of using binary mixtures of 1-pyrene butyric acid, 3,6-dimethylamino acridine, 4-acetamido-4''-isothiocyanatostilbene-2,2''-disulfonic acid, and rhodamine B isothiocyanate for the characterization and identification of microorganisms was tested with 98 yeast strains belonging ... More
Evidence for an electrogenic Na+-HCO3- symport in rat cardiac myocytes.
AuthorsAiello EA, Petroff MG, Mattiazzi AR, Cingolani HE
JournalJ Physiol
PubMed ID9729624
'1. The perforated whole-cell configuration of patch clamp and the pH fluorescent indicator SNARF were used to determine the electrogenicity of the Na+-HCO3- cotransport in isolated rat ventricular myocytes. 2. Switching from Hepes buffer to HCO3- buffer at constant extracellular pH (pHo) hyperpolarized the resting membrane potential (RMP) by 2.9 ... More
Effect of staphylococcal alpha-hemolysin upon anion transport in the rabbit erythrocyte.
AuthorsAustin JW, Fackrell HB
JournalBiochim Biophys Acta
PubMed ID6743657
'Equilibrium exchange of SO4(2-) was measured prior to and during hemolysis in rabbit erythrocytes exposed to staphylococcal alpha-hemolysin. The anion-transport protein of the rabbit erythrocyte has also been identified. Equilibrium exchange of SO4(2-) was measured by both efflux and influx of 35SO4(2-). The rate of influx of SO4(2-) in rabbit ... More
Intracellular Ca2+ does not activate the SITS-sensitive anion transporter in barnacle muscle.
AuthorsMatsumoto P, Russell JM
JournalBiochim Biophys Acta
PubMed ID3382644
'By means of the intracellular dialysis technique, we have measured 36Cl efflux from single barnacle muscle fibers and compared the effects of raising intracellular ionized calcium concentration [( Ca2+]i) to the effects of lowering intracellular pH (pHi). Lowering pHi by 1 unit or less resulted in a 20-fold stimulation of ... More
Chloride efflux measurements in mammalian skeletal muscle.
AuthorsHayward BS, Barchi RL
JournalMuscle Nerve
PubMed ID7374672
'A rapid sampling technique has been used to resolve the components of 36Cl efflux from isolated extensor digitorum longus (EDL) muscles of young rats. Four distinct fluxes with apparent rate constants of 4.40 +/- 0.02 min-1 (k1), 1.30 +/- 0.04 min-1 (k2), 0.24 +/- 0.04 min-1 (k3), and 0.048 +/- ... More
SITS: a covalently bound fluorescent retrograde tracer that does not appear to be taken up by fibers-of-passage.
AuthorsSchmued LC, Swanson LW
JournalBrain Res
PubMed ID6754001
Permeant anion activation of MgATPase activity in chromaffin granules. Evidence for direct coupling of proton and anion transport.
AuthorsPazoles CJ, Creutz CE, Ramu A, Pollard HB
JournalJ Biol Chem
PubMed ID6105158
Increase of degree of spermidine stimulation of polypeptide synthesis in the presence of phosphate.
AuthorsIgarashi K, Kashiwagi K, Kakegawa T, Aoki R, Hirose S
JournalArch Biochem Biophys
PubMed ID6263187
Effects of thyrotropin, acetazolamide, 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid, perchlorate, and ouabain on the distribution of iodide ion in cells and luminal fluid of turtle thyroid.
AuthorsChow SY, Yen-Chow YC, Woodbury DM
JournalEndocrinology
PubMed ID6274614
Catecholamine transport by isolated chromaffin granules. Influence of MgATP and a disulfonic stilbene on (R)-norepinephrine/epinephrine exchange and spontaneous epinephrine efflux.
AuthorsRamu A, Pazoles CJ, Creutz CE, Pollard HB
JournalJ Biol Chem
PubMed ID7451502
The nature of the membrane sites controlling anion permeability of human red blood cells as determined by studies with disulfonic stilbene derivatives.
AuthorsCabantchik ZI, Rothstein A
JournalJ Membr Biol
PubMed ID4667922
Chemical modification of membranes. I. Effects of sulfhydryl and amino reactive reagents on anion and cation permeability of the human red blood cell.
AuthorsKnauf PA, Rothstein A
JournalJ Gen Physiol
PubMed ID5559622
Effect of an inhibitor of anionic transport on fluid reabsorption from the proximal convoluted tubule of the rat [proceedings]
AuthorsGreen R, Greenwood SL
JournalJ Physiol
PubMed ID731573
Interactions of pHi and Ca2+i in vascular smooth muscle tissue.
AuthorsDaugirdas JT, Batlle DC
JournalJ Lab Clin Med
PubMed ID1593213
Sulfate transport in rabbit ileum: characterization of the serosal border anion exchange process.
AuthorsLangridge-Smith JE, Field M
JournalJ Membr Biol
PubMed ID7310859
Na+/H+ exchange and growth factor-induced cytosolic pH changes. Role in cellular proliferation.
AuthorsGrinstein S, Rotin D, Mason MJ
JournalBiochim Biophys Acta
PubMed ID2535787
Effects of amiloride and SITS on branchial ion fluxes in rainbow trout, Salmo gairdneri.
AuthorsPerry SF, Randall DJ
JournalJ Exp Zool
PubMed ID7276890
The effects of amiloride and SITS on branchial ion fluxes in rainbow trout were examined. Each drug was found significantly to inhibit both influx of Na+ and Cl- ions. It is postulated that the inhibitory effects on the contralateral exchange processes result from changes in gill epithelial cell pH. ... More
Coumarin-phalloidin: a new actin probe permitting triple immunofluorescence microscopy of the cytoskeleton.
AuthorsSmall JV, Zobeley S, Rinnerthaler G, Faulstich H
JournalJ Cell Sci
PubMed ID2458368
7-Diethylamino-3-(4-isothiocyanotophenyl)-4-methylcoumarin (CPITC) was coupled to amino-methyldithiolanophalloidin to produce a new phalloidin derivative, coumarin-phalloidin, fluorescent in the blue region of the spectrum. Coumarin-phalloidin binds to actin with around 100-fold less affinity than unconjugated phalloidin, but with enough avidity to make it a useful stain for actin filaments. Appropriate filter combinations permit ... More
Effects of the stilbene derivatives SITS and DIDS on intestinal ATPase activities.
AuthorsFaelli A, Tosco M, Orsenigo MN, Esposito G
JournalPharmacol Res Commun
PubMed ID6328545
4-Acetamido-4'-isothiocyano-2,2'-disulfonic stilbene (SITS) and 4,4'-diisothiocyano-2,2'-disulfonic stilbene (DIDS) effects have been tested both in the basolateral membranes (BLMs) of jejunum enterocytes and in the same intestinal tract, everted and incubated "in vitro". Total and (Na,K)-ATPase activities of BLMs are inhibited in a similar way by the two disulfonic stilbenes as well ... More
Calcium and calcium-dependent chloride currents generate action potentials in solitary cone photoreceptors.
AuthorsMaricq AV, Korenbrot JI
JournalNeuron
PubMed ID2483100
Vertebrate rod and cone photoreceptors hyperpolarize when illuminated. However, synaptic input from horizontal cells can depolarize cones and even elicit action potentials. Using the whole-cell tight-seal recording technique, we determined that, in solitary cones isolated from a lizard retina, action potentials can be generated by depolarizing current steps under conditions ... More
Characterization of Mg2+ efflux from human, rat and chicken erythrocytes.
AuthorsGünther T, Vormann J
JournalFEBS Lett
PubMed ID2753156
Net Mg2+ efflux from Mg2+-loaded, human, rat and chicken erythrocytes was measured in sucrose, NaCl and choline Cl medium. Thus, Na+-dependent (NaCl minus choline Cl) and Na+-independent Mg2+ efflux (in sucrose) were determined. Na+-dependent Mg2+ efflux amounted to 0.16, 8.9 and 1.57 mmol/l cells x 30 min, Na+-independent Mg2+ efflux ... More
The role of chloride ions on the transport of glycine in plasma membrane vesicles from glial cells.
AuthorsZafra F, Giménez C
JournalBiochim Biophys Acta
PubMed ID2923873
The high-affinity transport system for glycine in plasma membrane vesicles from C6 glioma cells is dependent on Na+ and also on the presence of Cl- in the incubation medium. This anion requirement is relatively specific for Cl-, since other anions are also capable of stimulating the glycine transport in the ... More
Anion requirement and effect of anion transport inhibitors on the response of vero cells to diphtheria toxin and modeccin.
AuthorsSandvig K, Olsnes S
JournalJ Cell Physiol
PubMed ID6707104
The anion requirement for toxic action of diphtheria toxin and modeccin was studied. In Cl- -free Hepes buffer made isotonic with mannitol, cells were insensitive to diphtheria toxin and modeccin. Just 2 mM NaCl was sufficient to obtain full toxic activity of modeccin, whereas 140 mM NaCl was required for ... More
Diphtheria toxin-induced channels in Vero cells selective for monovalent cations.
AuthorsSandvig K, Olsnes S
JournalJ Biol Chem
PubMed ID2457582
Ion fluxes associated with translocation of diphtheria toxin across the surface membrane of Vero cells were studied. When cells with surface-bound toxin were exposed to low pH to induce toxin entry, the cells became permeable to Na+, K+, H+, choline+, and glucosamine+. There was no increased permeability to Cl-, SO4(-2), ... More
Two embryonic, tissue-specific molecules identified by a double-label immunofluorescence technique for monoclonal antibodies.
AuthorsWessel GM, McClay DR
JournalJ Histochem Cytochem
PubMed ID3084626
We identify two tissue-specific molecules in the sea urchin embryo by an immunofluorescence technique capable of co-localizing monoclonal antibodies on the same tissue section. The technique uses monovalent Fab-fluorochrome conjugates as secondary reagents to avoid cross-talk of subsequent antibody probes. Using this technique, we show that two cell surface molecules ... More
Effect of calcium ionophore A23187 on electrogenic acid-base transport in turtle bladder. Inhibition of acidification and stimulation of alkalinization.
AuthorsEhrenspeck G
JournalBiochim Biophys Acta
PubMed ID6307361
Turtle bladders bathed on both surfaces with identical HCO3-/CO2-rich, Cl--free Na+ media and treated with ouabain and amiloride exhibit a transepithelial potential serosa electronegative to mucosa and a short-circuit current (Isc) which is a measure of the net luminal acidification rate. Addition of calcium ionophore A23187 (10 microM) to the ... More
Chloride self exchange in Ehrlich ascites cells. Inhibition by furosemide and 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid.
AuthorsAull F, Nachbar MS, Oppenheim JD
JournalBiochim Biophys Acta
PubMed ID921986
The effects of furosemide and 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS) on steady-state Cl- flux were studied in Ehrlich mouse ascites cells. At 10 mM, furosemide inhibited isotopically-determined Cl- flux by 86% without changing cell Cl- content, indicating that influx and efflux were depressed by the same amount. These results suggest that at ... More
Role of chloride/bicarbonate antiport in the control of cytosolic pH. Cell-line differences in activity and regulation of antiport.
AuthorsReinertsen KV, Tønnessen TI, Jacobsen J, Sandvig K, Olsnes S
JournalJ Biol Chem
PubMed ID3403517
Sodium-linked and sodium-independent HCO3-/Cl- antiport was measured under different conditions in a number of cell lines. Transport of HCO3- was estimated from its effect on intracellular pH (pHi) measured with the fluorescent probe 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein. The associated ion fluxes were estimated from the transport of 36Cl- and 22Na+. Na+-dependent and Na+-independent ... More
Mechanisms of stimulus-evoked intracellular acidification in frog nerve fibres.
AuthorsKhodorov B, Valkina O, Turovetsky V
JournalFEBS Lett
PubMed ID8137911
Measurements of cytoplasmic pH (pHi) in frog nerve fibers (sciatic nerve and its thin bundles) were performed by using fluorescein diacetate. Earlier it had been established that veratridine (VER) treatment of the nerve greatly enhances the stimulus-evoked intracellular acidification (SEIA) which becomes irreversible after blockade of the Na+/K+ pump with ... More
Thiocyanate inhibition of active chloride absorption in Aplysia intestine.
AuthorsGerencser GA
JournalBiochim Biophys Acta
PubMed ID6466680
This investigation was principally undertaken to examine the mechanism of active chloride absorption across the Aplysia californica intestine by using various inhibitors of ion transport. Isolated intestine, mounted between identical oxygenated sodium-free seawater solutions, maintained stable transmural potential differences (serosa negative) and short-circuit currents for several hours at 25 degrees ... More
Three-color fluorescence measurements on single cells excited at three laser wavelengths.
AuthorsSteinkamp JA, Stewart CC, Crissman HA
JournalCytometry
PubMed ID7056131
A three-laser flow cytometer for quantitative analysis and sorting of cells and microscopic particles has been developed and evaluated using argon- and krypton-ion lasers as excitation sources. Cells stained with three fluorescent dyes having different excitation spectra enter a flow chamber where they first pass through an electronic volume sensor ... More
Rapid purification and characterization of F1-ATPase of Vibrio parahaemolyticus.
AuthorsSakai Y, Kanazawa H, Tsuda M, Tsuchiya T
JournalBiochim Biophys Acta
PubMed ID2142893
The F1 portion of H(+)-translocating ATPase as purified from membrane vesicles of Vibrio parahaemolyticus by a rapid procedure. The whole purification process (from culture of cells to purification of the enzyme) could be completed in 1 day. The F1-ATPase consists of five subunits (alpha, beta, gamma, delta and epsilon) like ... More
Uptake and action of a disulphonic stilbene (SITS) in the perfused guinea-pig liver: a comparison with bromsulphthalein.
AuthorsRutishauser SC
JournalJ Physiol
PubMed ID6864568
1. Livers were perfused with a Krebs-Ringer bicarbonate buffer in a single-pass perfusion system. Bile secretion was maintained by infusion of secretin. 4-Acetamido-4'-isothiocyano-2,2'-stilbene disulphonic acid (SITS) was added to the perfusate to give concentrations ranging between 5 x 10(-6) and 10(-4) M. 2. SITS was extracted from the perfusate by ... More
The influence of pH and membrane potential on passive Na+ and K+ fluxes in human red blood cells.
AuthorsChipperfield AR, Shennan DB
JournalBiochim Biophys Acta
PubMed ID3011118
Passive (ouabain-insensitive) Na+ and K+ effluxes from human red blood cells were measured over the range pHo 6.2-8.5. On raising pHo, Na+ efflux increased and this was mainly attributable to the piretanide-sensitive component: K+ efflux likewise but attributable to both piretanide-sensitive and piretanide-insensitive components. On replacing Cl- with non-penetrating anions ... More
Regulation of intracellular pH in cultured hippocampal neurons by an amiloride-insensitive Na+/H+ exchanger.
AuthorsRaley-Susman KM, Cragoe EJ, Sapolsky RM, Kopito RR
JournalJ Biol Chem
PubMed ID1847131
Regulation of intracellular pH (pHi) in single cultured rat hippocampal neurons was investigated using the fluorescent pHi indicator dye bis-carboxyethylcarboxyfluorescein. Resting pHi was dependent on the presence of bicarbonate and external Na+ but was not altered significantly by removal of Cl- or treatment with the anion exchange inhibitor diisothiocyanatostilbene-2,2'-disulfonate. Recovery ... More
A relationship between anion transport and a structural transition of the human erythrocyte membrane.
AuthorsSnow JW, Vincentelli J, Brandts JF
JournalBiochim Biophys Acta
PubMed ID7025903
Scanning microcalorimetry was employed as an aid in examining some structural features of the anion transport system in red blood cell vesicles. Two structural transitions were previously shown to be sensitive to several covalent and non-covalent inhibitors of anion transport in red cells. In this study, these transitions were selectively ... More
Entry of diphtheria toxin linked to concanavalin A into primate and murine cells.
AuthorsGuillemot JC, Sundan A, Olsnes S, Sandvig K
JournalJ Cell Physiol
PubMed ID3844014
Diphtheria toxin linked by a disulfide bridge to concanavalin A was highly toxic to HeLa S3 and Vero cells, as well as to murine L cells. The cells could be protected with alpha-methyl mannoside, indicating that the conjugate binds mainly through its concanavalin A moiety. Treatment of Vero cells with ... More
Distributions of proteins and lipids in the erythrocyte membrane.
AuthorsRodgers W, Glaser M
JournalBiochemistry
PubMed ID8251477
Fluorescence digital imaging microscopy was used to characterize erythrocyte membrane domains. To investigate the role of specific proteins in forming lipid domains, erythrocyte ghosts were double-labeled with fluorescent phospholipids and a fluorescent label specific for band 3, the major integral membrane protein. Areas of enrichment, or domains, were observed for ... More
Stilbene disulfonic acids. CD4 antagonists that block human immunodeficiency virus type-1 growth at multiple stages of the virus life cycle.
AuthorsCardin AD, Smith PL, Hyde L, Blankenship DT, Bowlin TL, Schroeder K, Stauderman KA, Taylor DL, Tyms AS
JournalJ Biol Chem
PubMed ID2071607
The stilbene disulfonic acids 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), 4,4'-diisothiocyanatodihydrostilbene-2,2'-disulfonic acid and, 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid bound the variable-1 immunoglobulin-like domain of CD4 on JM cells. The interaction blocked the binding of the anti-CD4 monoclonal antibody OKT4A and the envelope glycoprotein gp120 of the human immunodeficiency virus type-1 (HIV-1). DIDS inhibited the acute infection ... More
Overexpression of a 123-kDa anion transport inhibitor binding protein and two cytoskeleton proteins in Drosophila Kc cell variants resistant to disulfonic stilbenes.
AuthorsSherwood AC, John-Alder K, Biessmann H, Sanders MM
JournalJ Biol Chem
PubMed ID2912986
Drugs of the disulfonic stilbene class, which inhibit anion transport in the cell membrane in many cell types, have been found to inhibit anion transport and cell growth in Drosophila Kc cells. Cell variants selected by a stepwise selection protocol for the ability to grow in the presence of the ... More
Intracellular calcium and pH alterations induced by tri-n-butyltin chloride in isolated rainbow trout hepatocytes: a flow cytometric analysis.
AuthorsReader S, Steen HB, Denizeau F
JournalArch Biochem Biophys
PubMed ID8037453
The effects of tri-n-butyltin chloride (TBT) on ionic homeostasis on isolated trout hepatocytes were investigated by flow cytometry (FCM), using the Ca(2+)-sensitive and pH-sensitive fluorescent probes Indo-1 and SNARF-1, respectively. Cell viability was monitored concurrently. Treatment of hepatocytes with 1 and 5 microM TBT caused a rapid and sustained elevation ... More
Macromolecular conjugates of transport inhibitors: new tools for probing topography of anion transport proteins.
AuthorsEidelman O, Yani P, Englert HC, Lang HG, Greger R, Cabantchik ZI
JournalAm J Physiol
PubMed ID2035616
Macromolecular-conjugated, water-soluble, membrane-impermeant compounds were designed and assessed as topological probes for chloride-transporting agencies. The novel compounds were derivatives of either disulfonic stilbene (DS) and benzylaminoethylsulfonate (BS), "classical" inhibitors of erythrocyte chloride-bicarbonate exchange, or of phenylanthranilates (PA), high-affinity blockers of epithelial chloride channels. Covalent reactive derivatives of various DS, BS, ... More
Intracellular pH and the stimulus-secretion coupling in insulin-producing RINm5F cells.
AuthorsJuntti-Berggren L, Rorsman P, Siffert W, Berggren PO
JournalBiochem J
PubMed ID1417791
The regulation of intracellular pH (pHi) and its role in the insulin-secretory process were evaluated, by using the clonal insulin-secreting cell line RINm5F. Glyceraldehyde, lactate and dihydroxyacetone decreased pHi, but only the first two released insulin. In the presence of extracellular Na+ the cells counteracted the acidification. Blocking the Na+/H+ ... More
Passive Ca transport in human red blood cell ghosts measured with entrapped arsenazo III.
AuthorsYingst DR, Hoffman JF
JournalJ Gen Physiol
PubMed ID6319541
The rate of Ca influx into ghosts containing arsenazo III changes with time, being most rapid during the first 5 min after Ca is added to the outside and declining thereafter. The rate of Ca influx is a nonlinear function of extracellular Ca and plateaus as the latter is increased ... More
Effects of glucose, chloromercuribenzene-p-sulphonic acid and 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid on phosphate efflux from pancreatic islets.
AuthorsAsplund K, Sehlin J, Taljedal IB
JournalBiochim Biophys Acta
PubMed ID228758
Collagenase-isolated pancreatic islets of non-inbred ob/ob mice, containing more than 90% beta-cells, were labelled with radioactive orthophosphate (32P or 33P) and then subjected to non-recirculating perifusion. The basal D-glucose concentration in the perifusion medium was 2.8 mM. When the concentration was suddenly raised to 5.6, 8.3 or 16.7 mM, D-glucose ... More
Affinity chromatography of Band 3, the anion transport protein of erythrocyte membranes.
AuthorsPimplikar SW, Reithmeier RA
JournalJ Biol Chem
PubMed ID3733694
Affinity chromatography of Band 3 was performed using a series of affinity matrices synthesized with various inhibitor ligands and spacer arms. Hydrophilic spacer arms greater than four atoms in length were essential for Band 3 binding. An affinity resin prepared by reacting 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate (Ki = 10 microM) with Affi-Gel 102 ... More
Kinetics of DIDS inhibition of swelling-activated K-Cl cotransport in low K sheep erythrocytes.
AuthorsDelpire E, Lauf PK
JournalJ Membr Biol
PubMed ID1593613
The inhibitory effect of various stilbene disulfonates was examined on the swelling-activated Cl-dependent K transport (K-Cl cotransport) in low K sheep erythrocytes. Both diisothiocyanatostilbenes H2DIDS and DIDS were found to be potent inhibitors. The DIDS concentration yielding 50% inhibition (IC50) of KCl cotransport was 60 microM in the absence of ... More
Effect of bicarbonate, pH, methazolamide and stilbenes on the intracellular potentials of cultured bovine corneal endothelial cells.
AuthorsJentsch TJ, Koch M, Bleckmann H, Wiederholt M
JournalJ Membr Biol
PubMed ID6325699
Micropuncture of cultured bovine corneal endothelial cells led to registrations stable for hours. Intracellular potentials were mainly in the range of -40 to -55 mV, average 46.3 +/- 0.6 mV (SEM). Changes of extracellular [HCO-3] led to voltage transients, their amplitude depending logarithmically on [HCO-3] with a mean slope of ... More
Evaluation of eight fluorochrome combinations for simultaneous DNA-protein flow analyses.
AuthorsStöhr M, Vogt-Schaden M, Knobloch M, Vogel R, Futterman G
JournalStain Technol
PubMed ID83691
Eight fluorescent dye combinations for simultaneous DNA-protein staining have been evaluated spectroscopically and flow microfluorometrically: propidium iodide (PI) with fluoresceinisothiocyanate (FITC), fluorescamine (FC), and dansylchloride (DANS); diamidinophenylindole (DAPII) with sulphorhodamin (SR101), tetramethylrhodamin isothiocyanate (TRITC), and nitrobenzodiazole (NBD); acriflavine (AF) with stilbene isothiocyanate sulphonic acid (SITS), and DAPI. Three different experimental ... More
Purification of bicarbonate-sensitive sperm adenylylcyclase by 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid-affinity chromatography.
AuthorsOkamura N, Tajima Y, Onoe S, Sugita Y
JournalJ Biol Chem
PubMed ID1655724
Anion transport inhibitors, such as SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid) and heparin, inhibit reversibly the bicarbonate-sensitive adenylylcyclase of porcine sperm plasma membrane. In the light of this, SITS- and heparin-affinity chromatographies were applied in order to purify sperm adenylylcyclase. SITS-Affi-Gel 102 binds proteins extracted from the porcine cauda epididymal sperm plasma membrane ... More
Measurement of the internal pH of mast cell granules using microvolumetric fluorescence and isotopic techniques.
AuthorsDe Young MB, Nemeth EF, Scarpa A
JournalArch Biochem Biophys
PubMed ID3579299
The intragranular pH of isolated mast cell granules was measured. Because of the minute amounts of isolated granules available, two techniques were developed by modifying aminoacridine fluorescence and [14C]methylamine accumulation techniques to permit measurements with microliter sample volumes. Granule purity was demonstrated by electron microscopy, ruthenium red exclusion, and biochemical ... More
Anion channel blockers inhibit lysosomal enzyme secretion from human neutrophils without affecting generation of superoxide anion.
AuthorsKorchak HM, Eisenstat BA, Hoffstein ST, Dunham PB, Weissmann G
JournalProc Natl Acad Sci U S A
PubMed ID6248861
The role of permeant anions in lysosomal enzyme secretion from human neutrophils was investigated by means of anion-channel-blocking agents: 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS), and pyridoxal phosphate. Lysosomal enzyme release from cytochalasin B-treated human neutrophils stimulated by immune complexes (bovine serum albumin and IgG anti-bovine serum albumin) was inhibited ... More
Kinetic analysis of the inhibition of anion transport in sarcoplasmic reticulum vesicles by a disulfonic stilbene derivative. Measurement of the change in chloride-diffusion potential by using a fluorescent cyanine dye.
AuthorsYamamoto N, Kasai M
JournalJ Biochem (Tokyo)
PubMed ID7275951
The characterization of the anion transport system in sarcoplasmic reticulum (SR) vesicles was performed by kinetic analysis of the reversible inhibition of gluconate efflux by a disulfonic stilbene derivative; 4-acetamido-4'-isothiocyano-stilbene-2,2'-disulfonate (SITS). The gluconate efflux in SR vesicles was measured by following the change in Cl- ion-diffusion potential due to the ... More
Effects of the anion transport inhibitor, SITS, on the proximal straight tubule of the rabbit perfused in vitro.
AuthorsBiagi BA
JournalJ Membr Biol
PubMed ID4093954
Conventional microelectrodes were used to study the effects of SITS (4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate) on the basolateral membrane potential Vbl of the superficial proximal straight tubule (PST) of the rabbit kidney perfused in vitro. Addition of 0.1 mM SITS to the bathing solution resulted in a slow and irreversible hyperpolarization of Vbl from ... More
Determination of membrane potential and cell volume by 19F NMR using trifluoroacetate and trifluoroacetamide probes.
AuthorsLondon RE, Gabel SA
JournalBiochemistry
PubMed ID2730869
The distribution of ionic species between intra- and extracellular compartments forms one basis for the determination of cell membrane potential. It is shown that fluorine-19 NMR studies of erythrocytes in the presence of trifluoroacetate, a stable, relatively nontoxic anion with pK = -0.3, provide a sensitive probe of membrane potential. ... More