Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef(每初始化 1 次测序运行)
Ion Torrent™
Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef(每初始化 1 次测序运行)
Ion 510 & Ion 520 & Ion 530 Kit-Chef(每初始化了解更多信息
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货号
数量
A34019
8 Reactions
货号 A34019
价格(CNY)
-
数量:
8 Reactions
Ion 510 & Ion 520 & Ion 530 Kit-Chef(每初始化 1 次测序运行)实现了高达 400 碱基读长文库的稳健、自动化模板制备和测序(使用 Ion Chef 系统和 Ion S5 或 Ion S5 XL 测序系统)。Ion 510 & Ion 520 & Ion 530 Kit 试剂盒包含预包装一次性模板和测序试剂卡,带有集成式样品追踪功能,从而为 8 个已加载芯片的测序提供自动化工作流程以及完整的运行可追溯性。本试剂盒容纳每初始化 1 次测序运行。有关每初始化 2 次测序运行(仅限 200 碱基读长应用),请参见货号 A34461。
Ion 510 & Ion 520 & Ion 530 Kit-Chef 工作流程: •为高达 400 碱基读长文库提供模板制备和芯片加载试剂 • 利用即用型、一次性试剂卡夹进行测序,简化研究 • 提供快速、简单的测序仪器设置,手动操作时间不到 15 分钟 • 利用数据质量和准确度新成果 • 与 Ion 510、Ion 520 和 Ion 530 芯片兼容,更大限度地提高灵活性
Ion S5 和 Ion S5 XL 测序仪搭配 Ion Chef 系统提供了可重现的测序工作流程,同时大大缩短了手动操作时间。选择将 Ion 510 & Ion 520 & Ion 530 试剂盒与 Ion 510 Chip 试剂盒(每芯片高达 3 百万次读取)、Ion 520 Chip 试剂盒(每芯片高达 5 百万次读取)或 Ion 530 Chip 试剂盒(每芯片高达 20 百万次读取)配对,实现总通量的灵活性。如要获取更大灵活性,使用 Torrent Suite 软件 5.6 及更高版本时,可将不同芯片类型组合在一个 Ion Chef 运行中。对于从头研究应用,在制备好的文库中加入 Ion S5 校准标准品,以提高碱基识别准确度。
仅供科研使用。不可用于诊断程序。
规格
产品类型Chef 试剂盒
数量8 Reactions
运输条件经批准可在室温下或置于湿冰上运输
通量400 碱基读长文库
产品线Ion Chef™,Ion Torrent™
软件类型Torrent Suite 软件 5.6
Unit SizeEach
内容与储存
• Ion S5 Chef 用品,室温储存 • Ion S5 Chef 溶液,室温储存 • Ion 510 和 Ion 520 和 Ion 530 Chef 试剂,-5 至 -30°C 下储存 • Ion S5 测序溶液,室温储存 • Ion S5 测序试剂,-5 至 -30°C 下储存
常见问题解答 (FAQ)
Why did my Ion Chef Instrument stop during a run?
- The instrument may have encountered an error. We recommend recording the error shown on the instrument display, then tapping OK. For further assistance, contact Technical Support at techsupport@thermofisher.com.
- A consumable may have been improperly loaded. We recommend ensuring that all consumables are correctly loaded. For instructions, see the user guide for your templating kit. For further assistance, contact Technical Support at techsupport@thermofisher.com.
Why is the door of the Ion Chef Instrument not opening?
- An obstruction may be present on or around the door mechanism. We recommend removing the obstruction blocking the door, then resuming normal instrument operation.
- The instument may be experiencing a hardware or software error. We recommend contacting Technical Support at techsupport@thermofisher.com.C8
Why does the Ion Chef Instrument not display the home screen when powered on?
Multiple causes are possible. We recommend powering off the instrument, waiting for 30 secs and then powering on the instrument. If you continue to experience the issue, contact Technical Support at techsupport@thermofisher.com.
For the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay, what are the analysis workflows in Ion Reporter Software?
There are two analysis workflows available:
1. Oncomine TagSeq Lung v2 Liquid Biopsy - w2.0 - Single Sample: Detects and annotates low-frequency variants including SNPs/Indels (down to 0.1% limit of detection), Fusions, and CNVs from targeted nucleic acid libraries (DNA & RNA) from the Oncomine Lung Cell‑Free Total Nucleic Acid Research Assay. This is compatible with DNA & RNA purified from cell-free total nucleic acids.
2. Oncomine TagSeq Lung v2 Tumor - w2.0 - Single Sample: Detects and annotates low-frequency variants including SNPs/Indels (down to 0.5% limit of detection), fusions, and CNVs from targeted nucleic acid libraries (DNA & RNA) from the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay. Due to deamination events caused by the FFPE process, the minimum alternative allele frequency is set to 0.3%. This makes it compatible with DNA & RNA purified from FFPE tumor tissue as well as fresh frozen tumor tissue.
Are there any BED files for the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay?
Yes, there is a specific BED file and Hotspot file for the Oncomine Lung Cell-Free Total Nucleic Acid Research Assay. Please contact your local Field Application Specialist (FAS) or Clinical Application Consultant (CAC) to request the BED files.