Ion 550™ Chip Kit, 8 Chips - FAQs

View additional product information for Ion 550™ Chip Kit - FAQs (A34537, A34538)

12 product FAQs found

Why did my Ion Chef Instrument stop during a run?

- The instrument may have encountered an error. We recommend recording the error shown on the instrument display, then tapping OK. For further assistance, contact Technical Support at techsupport@thermofisher.com.
- A consumable may have been improperly loaded. We recommend ensuring that all consumables are correctly loaded. For instructions, see the user guide for your templating kit. For further assistance, contact Technical Support at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Why is the door of the Ion Chef Instrument not opening?

- An obstruction may be present on or around the door mechanism. We recommend removing the obstruction blocking the door, then resuming normal instrument operation.
- The instument may be experiencing a hardware or software error. We recommend contacting Technical Support at techsupport@thermofisher.com.C8

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Why does the Ion Chef Instrument not display the home screen when powered on?

Multiple causes are possible. We recommend powering off the instrument, waiting for 30 secs and then powering on the instrument. If you continue to experience the issue, contact Technical Support at techsupport@thermofisher.com.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How long can my loaded Ion 550 chip be stored prior to running on the sequencer?

Remove and sequence chips as soon as possible after the Ion Chef System finishes loading them. If you cannot sequence a loaded chip immediately, store it in a chip storage container at 4 degrees C until you are ready to run it (up to 24 hours maximum).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Why do my Ion 550 chip "Reagent Check" traces look different from those seen with the other Ion chips (510, 520, 530, and 540)?

This is because the new chip surface and the way the instrument reads the Ion 550 chip are different from the Ion 540/530/520/510 chips.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many Oncomine Comprehensive Assay v3 libraries may be pooled on a single Ion 550 chip?

The Oncomine Comprehensive Assay v3 is validated with 32 libraries per Ion 550 chip (16 samples DNA and RNA).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many Ion AmpliSeq Human Exome libraries can be pooled on a single Ion 550 chip?

The Ion 550 workflow supports 3-4 Ion AmpliSeq Human Exome libraries per run.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the shipping and storage conditions for the Ion 550 chip?

The Ion 550 chip is shipped at room temperature and should be stored at room temperature.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Which instrument platforms can I use with the Ion 550 chip?

The Ion 550 Chip is compatible with the Ion GeneStudio S5 Plus System, Ion GeneStudio S5 Prime System, and Ion S5 XL System. The Ion GeneStudio S5 System and Ion S5 System do not have enough computing power to work with the Ion 550 chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I perform templating with either the Ion OneTouch 2 System or the Ion Chef System when using the Ion 550 chip for sequencing?

The Ion 550 chip workflow is only supported with the Ion Chef System for templating.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the largest length I can sequence with an Ion 550 chip?

Up to 200 bp libraries (500 flows) are supported on the Ion 550 chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reads can I expect with an Ion 550 chip?

You may expect 100-130 million reads with an Ion 550 chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.