ReproSeq™ PGS Kit with Ion 520™ Chips (24 samples/run) - FAQs

查看更多产品信息 ReproSeq™ PGS Kit with Ion 520™ Chips (24 samples/run) - FAQs (A34900)

7 个常见问题解答

How many total samples can I process with the Ion ReproSeq PGS Kit with Ion 520 Chips?

96 samples in total (4 runs with 24 samples per run) can be processed with the Ion ReproSeq PGS Kit with Ion 520 Chips.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many samples per run can I process with the Ion ReproSeq PGS Kit with Ion 520 Chips?

24 samples per run can be processed with the Ion ReproSeq PGS Kit with Ion 520 Chips.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What does the Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run) include, and what are the storage conditions for each component?

*Ion SingleSeq 96 Kit, 1 box, -10 to -30 degrees C
*Ion S5 ExT Chef Supplies, 4 boxes, 15-30 degrees C
*Ion S5 Chef Solutions, 4 cartridges, 15-30 degrees C
*Ion S5 ExT Chef PGS Reagents, 4 cartridges, -10 to -30 degrees C
*Ion S5 ExT Sequencing Solutions, 1 box, 15-30 degrees C
*Ion S5 ExT Sequencing Reagents, 4 cartridges, -10 to -30 degrees C
*Ion 520 Chip Kit, 4 chips, 15-30 degrees C

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Does the Ion SingleSeq 96 Kit include reagents for template preparation, sequencing, and chips?

No, the Ion SingleSeq 96 Kit only includes reagents to prepare the library. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion SingleSeq 96 Kit?

The Ion SingleSeq 96 Kit includes reagents to extract, amplify, and barcode genomic DNA for preparation of libraries from up to 96 samples. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Where can I find the sequences for the IonCode barcodes?

The sequences for the barcodes can be found in the Barcodes section of your Torrent Browser. You can also download a .csv file containing the full list of sequences in that set.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Are the Ion ReproSeq PGS Kit subkits available separately?

Yes, the Ion SingleSeq Kit (Cat. No. A28955, 24 reactions/kit), or Ion SingleSeq 96 Kit (Cat. No. A34763, 96 reactions/kit) are available separately.

The Ion ReproSeq PGS Kits - Ion S5 System, are also available separately and include reagents to prepare templates and load 4 chips on the Ion Chef Instrument, and sequence the chips on the Ion S5 or Ion S5 XL Sequencer.
- Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run), Cat. No. A34900
- Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run), Cat. No. A34901

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.