ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) - FAQs

384 samples in total (4 runs with 96 samples/run) can be processed with the Ion ReproSeq PGS Kit with Ion 530 Chips.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

96 samples per run can be processed with the Ion ReproSeq PGS Kit with Ion 530 Chips.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

*Ion SingleSeq 96 Kit, 4 boxes, -10 to -30 degrees C
*Ion S5 ExT Chef Supplies, 4 boxes, 15-30 degrees C
*Ion S5 Chef Solutions, 4 cartridges, 15-30 degrees C
*Ion S5 ExT Chef PGS Reagents, 4 cartridges, -10 to -30 degrees C
*Ion S5 ExT Sequencing Solutions, 1 box, 15-30 degrees C
*Ion S5 ExT Sequencing Reagents, 4 cartridges, -10 to -30 degrees C
*Ion 530 Chip Kit, 4 chips, 15-30 degrees C

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

No, the Ion SingleSeq 96 Kit only includes reagents to prepare the library. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Ion SingleSeq 96 Kit includes reagents to extract, amplify, and barcode genomic DNA for preparation of libraries from up to 96 samples. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The sequences for the barcodes can be found in the Barcodes section of your Torrent Browser. You can also download a .csv file containing the full list of sequences in that set.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Yes, the Ion SingleSeq Kit (Cat. No. A28955, 24 reactions/kit), or Ion SingleSeq 96 Kit (Cat. No. A34763, 96 reactions/kit) are available separately.

The Ion ReproSeq PGS Kits - Ion S5 System, are also available separately and include reagents to prepare templates and load 4 chips on the Ion Chef Instrument, and sequence the chips on the Ion S5 or Ion S5 XL Sequencer.
- Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run), Cat. No. A34900
- Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run), Cat. No. A34901

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.