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Citations & References (15)
Invitrogen™
Annexin V Conjugates for Apoptosis Detection
Detect early stages of apoptosis with Annexin V stand-alone Alexa Fluor, APC, Pacific Blue, PE, FITC, and biotin conjugates using flow cytometry.
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| Catalog Number | Excitation/Emission | Flow Cytometer Laser Lines | Conjugate |
|---|---|---|---|
| A35110 | 650/660 | 633-637 | APC (Allophycocyanin) |
| A13201 | 495/519 | 488 | Alexa Fluor 488 |
| A13199 | 494/518 | 488 | FITC |
| A13202 | 578/603 | 532, 561 | Alexa Fluor 568 |
| A13203 | 590/617 | 532 | Alexa Fluor 594 |
| A13204 | Biotin-X | ||
| A23202 | 346/442 | UV | Alexa Fluor 350 |
| A23204 | 650/665 | 633-637 | Alexa Fluor 647 |
| A35108 | 555/565 | 532, 561 | Alexa Fluor 555 |
| A35109 | 679/702 | 633-637 | Alexa Fluor 680 |
| A35111 | 565/578 | 488, 532, 561 | PE |
| A35122 | 410/455 | 405 | Pacific Blue |
Catalog number A35110
Price (CNY)
4,198.00
Online Exclusive
Ends: 31-Dec-2025
5,577.00Save 1,379.00 (25%)
Each
Excitation/Emission:
650/660
Flow Cytometer Laser Lines:
633-637
Conjugate:
APC (Allophycocyanin)
Price (CNY)
4,198.00
Online Exclusive
Ends: 31-Dec-2025
5,577.00Save 1,379.00 (25%)
Each
Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry.
Annexin V has a high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. Because of this affinity, fluorescently labeled annexin V reagents are commonly used in apoptosis research.
Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.
In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.
The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes.
The benefits of our annexin V conjugates include:
• Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals
• Conjugates for all available lasers
• Available as stand-alone reagents or easy-to-use kits
Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.
Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.
In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.
The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes.
The benefits of our annexin V conjugates include:
• Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals
• Conjugates for all available lasers
• Available as stand-alone reagents or easy-to-use kits
Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed
DescriptionAnnexin V, APC conjugate (APC Annexin V) (replaces Annexin product- AnnexinV05)
Excitation/Emission650/660
Flow Cytometer Laser Lines633-637
For Use With (Equipment)Flow Cytometer
Kit ContentsContains 1 vial of annexin V, APC conjugate.
No. of Reactions50
Product TypeAnnexin V conjugate
Quantity250 μL
Shipping ConditionWet Ice
ConjugateAPC (Allophycocyanin)
Unit SizeEach
Contents & Storage
Store in refrigerator (2°C to 8°C) and protect from light.
Frequently asked questions (FAQs)
I trypsinized my adherent cells and labeled with annexin V, and now my flow data is showing a high percentage of apoptotic cells even for control, untreated cells. What is the problem?
Can I detect annexin V staining in an imaging assay?
When should I stain adherent cells with annexin V for flow cytometric analysis? Before or after I trypsinize them?
Can I fix my cells after annexin V labeling?
I am trying to label adherent cells with annexin V and am finding that everything is getting labeled. How can I fix this?
Citations & References (15)
Citations & References
Abstract
Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy.
Journal:Nat Protoc
PubMed ID:17853886
'Annexin V and Sytox Green are widely used markers to evaluate apoptosis in various cell types using flow cytometry and fluorescent microscopy. Recently, a novel fluoroprobe MitoSOX Red was introduced for selective detection of superoxide in the mitochondria of live cells and was validated for confocal microscopy and flow cytometry.
Cathepsin D and H2O2 stimulate degradation of thioredoxin-1: implication for endothelial cell apoptosis.
Journal:J Biol Chem
PubMed ID:16263712
'Cathepsin D (CatD) is a lysosomal aspartic proteinase and plays an important role in the degradation of proteins and in apoptotic processes induced by oxidative stress, cytokines, and aging. All of these stimuli are potent inducers of endothelial cell apoptosis. Therefore, we investigated the role of CatD in endothelial cell
Role of superoxide, nitric oxide, and peroxynitrite in doxorubicin-induced cell death in vivo and in vitro.
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:19286953
'Doxorubicin (DOX) is a potent available antitumor agent; however, its clinical use is limited because of its cardiotoxicity. Cell death is a key component in DOX-induced cardiotoxicity, but its mechanisms are elusive. Here, we explore the role of superoxide, nitric oxide (NO), and peroxynitrite in DOX-induced cell death using both
FTY720 increases CD74 expression and sensitizes mantle cell lymphoma cells to milatuzumab-mediated cell death.
Journal:Blood
PubMed ID:22042694
'Mantle cell lymphoma (MCL) is an aggressive B-cell malignancy with a short median survival despite multimodal therapy. FTY720, an immunosuppressive drug approved for the treatment of multiple sclerosis, promotes MCL cell death concurrent with down-modulation of phospho-Akt and cyclin D1 and subsequent cell-cycle arrest. However, the mechanism of FTY720-mediated MCL
Flow cytometry-based apoptosis detection.
Journal:Methods Mol Biol
PubMed ID:19609746
'An apoptosing cell demonstrates multitude of characteristic morphological and biochemical features, which vary depending on the stimuli and the cell type. The gross majority of classical apoptotic hallmarks can be rapidly examined by flow and image cytometry. Cytometry thus became a technology of choice in diverse studies of cellular demise.