Visualization of lipid domains in giant unilamellar vesicles using an environment-sensitive membrane probe based on 3-hydroxyflavone.
AuthorsKlymchenko AS, Oncul S, Didier P, Schaub E, Bagatolli L, Duportail G, Mély Y,
JournalBiochim Biophys Acta
PubMed ID19027712
'We characterized the recently introduced environment-sensitive fluorescent membrane probe based on 3-hydroxyflavone, F2N12S, in model lipid membranes displaying liquid disordered (Ld) phase, liquid ordered (Lo) phase, or their coexistence. Steady-state fluorescence studies in large unilamellar vesicles show that the probe dual emission drastically changes with the lipid bilayer phase, which ... More
Monitoring biophysical properties of lipid membranes by environment-sensitive fluorescent probes.
AuthorsDemchenko AP, Mély Y, Duportail G, Klymchenko AS,
JournalBiophys J
PubMed ID19413953
We review the main trends in the development of fluorescence probes to obtain information about the structure, dynamics, and interactions in biomembranes. These probes are efficient for studying the microscopic analogs of viscosity, polarity, and hydration, as well as the molecular order, environment relaxation, and electrostatic potentials at the sites ... More
Excited state proton transfer and solvent relaxation of a 3-hydroxyflavone probe in lipid bilayers.
AuthorsDas R, Klymchenko AS, Duportail G, Mély Y,
JournalJ Phys Chem B
PubMed ID18729506
The photophysics of a ratiometric fluorescent probe, N-[[4'- N, N-diethylamino-3-hydroxy-6-flavonyl]methyl]- N-methyl- N-(3-sulfopropyl)-1-dodecanaminium, inner salt (F2N12S), incorporated into phospholipid unilamellar vesicles is presented. The reconstructed time-resolved emission spectra (TRES) unravels a unique feature in the photophysics of this probe. TRES exhibit signatures of both an excited-state intramolecular proton transfer (ESIPT) and ... More
Fluorescent biomembrane probe for ratiometric detection of apoptosis.
AuthorsShynkar VV, Klymchenko AS, Kunzelmann C, Duportail G, Muller CD, Demchenko AP, Freyssinet JM, Mely Y,
JournalJ Am Chem Soc
PubMed ID17256940
Herein, we developed the first ratiometric fluorescent probe for apoptosis detection. This probe incorporates selectively into the outer leaflet of the cell plasma membrane and senses the loss of the plasma membrane asymmetry occurring during the early steps of apoptosis. The high specificity to the plasma membranes was achieved by ... More
Death receptor 5 is activated by fucosylation in colon cancer cells.
AuthorsZhang B, van Roosmalen IAM, Reis CR, Setroikromo R, Quax WJ
JournalFEBS J
PubMed ID30589515
'The remarkable pro-apoptotic properties of tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) have led to considerable interest in this protein as a potential anticancer therapeutic. However, TRAIL is largely ineffective in inducing apoptosis in certain cancer cells, and the mechanisms underlying this selectivity are unknown. In colon adenocarcinomas, posttranslational modifications ... More
Histone Deacetylase Inhibitors Sensitize TRAIL-Induced Apoptosis in Colon Cancer Cells.
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered as a promising anti-cancer therapeutic. However, many cancers have been found to be or to become inherently resistant to TRAIL. A combination of epigenetic modifiers, such as histone deacetylase inhibitors (HDACi's), with TRAIL was effective to overcome TRAIL resistance in some cancers. ... More