Pierce™ DSG, No-Weigh™ Format
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Pierce™ DSG, No-Weigh™ Format
Thermo Scientific™

Pierce™ DSG, No-Weigh™ Format

Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surfaceRead more
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Catalog NumberQuantity
A3539210 x 1 mg
2059350 mg
Catalog number A35392
Price (CNY)
1,447.00
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Ends: 31-Dec-2025
2,111.00
Save 664.00 (31%)
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Quantity:
10 x 1 mg
Price (CNY)
1,447.00
Online Exclusive
Ends: 31-Dec-2025
2,111.00
Save 664.00 (31%)
Each
Add to cart
Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Features of disuccinimidyl glutarate:

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
• Membrane-permeable, allowing for intracellular crosslinking
• Can increase crosslinking efficiency compared to DSS in some applications

Product References
Crosslinker Application Guide -- search for recent literature references for this product

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityYes
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)326.26
PEGylatedNo
Product LineNo-Weigh™, Pierce™
Quantity10 x 1 mg
Reactive MoietyNHS Ester
Shipping ConditionWet Ice
SolubilityDMF, DMSO
Spacer Arm Length7.7 Å
Water SolubleNo
Chemical ReactivityAmine-Amine
CleavableNo
Crosslinker TypeHomobifunctional
FormatStandard, Single-use
Product TypeCrosslinker
SpacerShort (<10 Å)
Unit SizeEach
Contents & Storage
Upon receipt store desiccated at 2 to 8°C.

Frequently asked questions (FAQs)

Can you provide the shelf-life for DSG (disuccinimidyl glutarate)?

DSG (disuccinimidyl glutarate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How should I dissolve and store DSG, No-Weigh Format?

DSG is an NHS-ester that hydrolyzes easily and is moisture-sensitive. For optimal results, we recommend storing DSG powder desiccated at 4 degrees C. To avoid moisture condensation onto the product, the vial must be equilibrated to room temperature before opening. We recommend resuspending DSG in DMSO, Anhydrous (Cat. No. D12345) right before use and not storing the solution.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How does DSP work as a crosslinker?

DSP is a homobifunctional, water soluble, cleavable, amine-reactive cross-linker. The amine-reactive ends are NHS-esters. This group reacts with primary amines, in proteins that translates to lysine groups and N-terminus amines. It reacts best in the pH range of 7.5 to 8.5, this ensures the amines are deprotonated and it limits the hydrolysis of the ester at higher pHs. NHS-esters are best used in a heavy molar excess over the number of amines present. This helps to overcome the competing hydrolysis of the ester to an inactive form.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How is the DSP cross-linking reaction quenched?

1M Tris (or glycine), pH 7.5 can be added to the reaction mixture to a final concentration of approximately 20-50 mM.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How is DSP cleaved?

The disulfIde bond in DSP's 12Å spacer arm can be cleaved by at least three different methods. The first is by incubating the crosslinker in 10-50 mM DTT for 30 minutes at 37 degrees C. Secondly, the disulfIde can be reduced by incubation in 5% 2-Mercaptoethanol (2-ME) in SDS-PAGE sample buffer at 100 degrees C for 5 minutes. Finally, the “-S-S-” bond can be cleaved by incubation in 100 mM sodium borohydride at room temperature for 30-60 minutes. Other reducing agents such as TCEP-HCl or 2-Mercaptoethylamine (2-MEA) should also work, however these applications have not been referenced.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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