SimpliAmp™ Thermal Cycler Extended Warranty Package, Instrument + 3 yr ABRC warranty - FAQs

查看更多产品信息 SimpliAmp™ Thermal Cycler - FAQs (A24811, A35422)

2 个常见问题解答

Can I purchase the sample block for the SimpliAmp Thermal Cycler (Cat. No. A24811) as a standalone product? Is it replaceable/interchangeable?

Unfortunately, we do not sell the sample block for the SimpliAmp Thermal Cycler separately. Only the ProFlex series and the Automated series of thermal cyclers have interchangeable blocks that we sell separately.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.