Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?
AuthorsHalliwell B, Whiteman M
JournalBr J Pharmacol
PubMed ID15155533
'Free radicals and other reactive species (RS) are thought to play an important role in many human diseases. Establishing their precise role requires the ability to measure them and the oxidative damage that they cause. This article first reviews what is meant by the terms free radical, RS, antioxidant, oxidative ... More
Externally applied pressure activates pancreatic stellate cells through the generation of intracellular reactive oxygen species.
AuthorsAsaumi H, Watanabe S, Taguchi M, Tashiro M, Otsuki M,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID17761838
'Local tissue pressure is higher in chronic pancreatitis than in the normal pancreas. We reported recently that pressure application induces synthesis of extracellular matrix (ECM) and cytokines in pancreatic stellate cells (PSCs) and that epigallocatechin gallate (EGCG), a potent antioxidant, inhibits the transformation of PSCs from quiescent to activated phenotype ... More
Fluorescence probes used for detection of reactive oxygen species.
AuthorsGomes A, Fernandes E, Lima JL,
JournalJ Biochem Biophys Methods
PubMed ID16297980
'Endogenously produced pro-oxidant reactive species are essential to life, being involved in several biological functions. However, when overproduced (e.g. due to exogenous stimulation), or when the levels of antioxidants become severely depleted, these reactive species become highly harmful, causing oxidative stress through the oxidation of biomolecules, leading to cellular damage ... More
Monitoring singlet oxygen and hydroxyl radical formation with fluorescent probes during photodynamic therapy.
AuthorsPrice M, Reiners JJ, Santiago AM, Kessel D,
JournalPhotochem Photobiol
PubMed ID19508643
'Singlet oxygen (1O2) is the primary oxidant generated in photodynamic therapy (PDT) protocols involving sensitizers resulting in type II reactions. 1O2 can give rise to additional reactive oxygen species (ROS) such as the hydroxyl radical (*OH). The current study was designed to assess 3''-p-(aminophenyl) fluorescein (APF) and 3''-p-(hydroxyphenyl) fluorescein (HPF) ... More
Myeloperoxidase is a key regulator of oxidative stress mediated apoptosis in myeloid leukemic cells.
AuthorsNakazato T, Sagawa M, Yamato K, Xian M, Yamamoto T, Suematsu M, Ikeda Y, Kizaki M,
JournalClin Cancer Res
PubMed ID17875773
'PURPOSE: We reported previously that reactive oxygen species (ROS) are key mediators of apoptosis induced by a polyphenol, (-)-epigallocatechin-3-gallate (EGCG), in myeloid leukemic cells. This study aimed to further examine the mechanism of ROS-mediated apoptosis induced by EGCG and its relationship to the heme enzyme myeloperoxidase (MPO). EXPERIMENTAL DESIGN: We ... More
On the use of fluorescence probes for detecting reactive oxygen and nitrogen species associated with photodynamic therapy.
AuthorsPrice M, Kessel D,
JournalJ Biomed Opt
PubMed ID21054079
Fluorescent probes are frequently employed for the detection of different reactive oxygen and nitrogen species formed during the irradiation of photosensitized cells and tissues. Investigators often interpret the results in terms of information provided with the different probes without examining specificity or determinants of fluorogenic reactions. We examine five fluorescent ... More
Morphological and flow-cytometric analysis of haemin-induced human neutrophil activation: implications for transfusion-related acute lung injury.
AuthorsKono M, Saigo K, Takagi Y, Kawauchi S, Wada A, Hashimoto M, Sugimoto T, Takenokuchi M, Morikawa T, Funakoshi K,
JournalBlood Transfus
PubMed ID22790260
Transfusion-related acute lung injury (TRALI) is associated with vascular endothelial cell injury following neutrophil activation. Recently, it has been suggested that haem-related molecules induce activation of neutrophils and that erythrocyte-derived substances contained in blood preparations are involved in TRALI. We observed the morphological effects and reactive oxygen species (ROS) production ... More
A novel flow-injection analysis system for evaluation of antioxidants by using sodium dichloroisocyanurate as a source of hypochlorite anion.
AuthorsIchiba H, Hanami K, Yagasaki K, Tanaka M, Ito H, Fukushima T,
JournalDrug Discov Ther
PubMed ID22460428
A flow injection analysis (FIA) system for evaluation of the antioxidant activity of a compound capable of scavenging a hypochlorite anion (OCl?), one of the reactive oxygen species (ROS), was developed. Aminophenyl fluorescein (APF), a fluorescence indicator of ROS, was mixed manually with the test compounds and the mixed solution ... More
Comparison of fluorescence-based techniques for the quantification of particle-induced hydroxyl radicals.
AuthorsCohn CA, Simon SR, Schoonen MA,
JournalPart Fibre Toxicol
PubMed ID18307787
ABSTRACT: BACKGROUND: Reactive oxygen species including hydroxyl radicals can cause oxidative stress and mutations. Inhaled particulate matter can trigger formation of hydroxyl radicals, which have been implicated as one of the causes of particulate-induced lung disease. The extreme reactivity of hydroxyl radicals presents challenges to their detection and quantification. Here, ... More
Regulation of cholesterol and sphingomyelin metabolism by amyloid-beta and presenilin.
AuthorsGrimm MO, Grimm HS, Pätzold AJ, Zinser EG, Halonen R, Duering M, Tschäpe JA, De Strooper B, Müller U, Shen J, Hartmann T
JournalNat Cell Biol
PubMed ID16227967
Amyloid beta peptide (Abeta) has a key role in the pathological process of Alzheimer's disease (AD), but the physiological function of Abeta and of the amyloid precursor protein (APP) is unknown. Recently, it was shown that APP processing is sensitive to cholesterol and other lipids. Hydroxymethylglutaryl-CoA reductase (HMGR) and sphingomyelinases ... More
Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species.
AuthorsSetsukinai K, Urano Y, Kakinuma K, Majima HJ, Nagano T
JournalJ Biol Chem
PubMed ID12419811
We designed and synthesized 2-[6-(4'-hydroxy)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (HPF) and 2- [6-(4'-amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid (APF) as novel fluorescence probes to detect selectively highly reactive oxygen species (hROS) such as hydroxyl radical (*OH) and reactive intermediates of peroxidase. Although HPF and APF themselves scarcely fluoresced, APF selectively and dose-dependently afforded a strongly fluorescent compound, ... More
Reduction of measurement noise in a continuous glucose monitor by coating the sensor with a zwitterionic polymer.
Authors
JournalNat Biomed Eng
PubMed ID30931173
TRPC6 channel translocation into phagosomal membrane augments phagosomal function.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID26604306
Boronate-based fluorescent probes: imaging hydrogen peroxide in living systems.
Authors
JournalMethods Enzymol
PubMed ID23791092
Alternative oxidase impacts the plant response to biotic stress by influencing the mitochondrial generation of reactive oxygen species.
Authors
JournalPlant Cell Environ
PubMed ID22978428
Nickel-induced epithelial-mesenchymal transition by reactive oxygen species generation and E-cadherin promoter hypermethylation.
Authors
JournalJ Biol Chem
PubMed ID22648416
Colony stimulating factor-1 receptor is a central component of the foreign body response to biomaterial implants in rodents and non-human primates.