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High Select™ Top14 Abundant Protein Depletion Resin, 50 mL - FAQs

High Select™ Top14 Abundant Protein Depletion Resin, 50 mL - FAQs

查看更多产品信息 High Select™ Depletion Spin Columns - FAQs (A36369, A36368, A36367, A36372, A36371, A36370, A36366, A36365)

8 个常见问题解答

Is there a 96-well format available for the Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369)?

No. The Top14 Abundant Protein Depletion Mini Spin Columns (Cat. No. A36369) do not come in a 96-well format. However, we do have a Top14 Abundant Protein Depletion Resin (Cat. No. A36372) that can be purchased as a stand-alone product.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After depletion of abundant proteins from my sample, I still see albumin and other abundant proteins after LC-MS analysis. Did the depletion work?

Depletion does not remove all abundant proteins from the sample. Even with 99% depletion of a specific plasma protein, highly abundant proteins will still be the most abundant proteins in the sample detected by MS analysis. However, depletion significantly decreases the most abundant plasma proteins enabling identification of lower abundant proteins.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I proceed to downstream mass spec sample preparation after abundant plasma protein depletion?

After the depletion, samples are significantly diluted in buffer. Depleted samples should be concentrated by speed vac drying, solvent precipitation, or diafiltration. Samples dried using a speedvac are directly compatible with the EasyPep kit chemistry after resuspension using the Lysis Solution.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What is the maximum loading capacity for the High Select Depletion columns?

Mini columns can deplete up to 10 µL (600 µg) of plasma or serum; midi columns can deplete up to 100 µL (6000 µg) of plasma or serum.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What is the percentage of albumin that is removed using abundant protein depletion columns?

More than 99% of albumin can be depleted when columns use the recommended sample to resin ratio.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I assess efficiency and reproducibility of abundant protein depletion in plasma samples?

ELISA and mass spectrometry analysis are often used for evaluation of depletion performance. Protein yields after depletion can be measured using the BCA assay.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What formats of the High Select Top 14 Abundant Protein Depletion Resin do you offer?

The High Select Top 14 Abundant Protein Depletion Resin is offered in 3 formats:

a) Resin alone: High Select Top 14 Abundant Protein Depletion Resin (Cat. No. A36372)
b) High Select Top 14 Abundant Protein Depletion Mini Spin Columns (Cat. Nos. A36369, A36370) that can process a maximum of 10 µL of human serum or plasma
c) High Select Top 14 Abundant Protein Depletion Midi Spin Columns (Cat. No. A36371) that can process a maximum of 100 µL of human serum or plasma. For serum samples containing abnormally high amounts of albumin or IgG, the sample load may need to be reduced.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Which proteins are removed by High Select Top 14 Abundant Protein Depletion Resin?

The High Select Top14 Abundant Protein Depletion Resin uses immobilized antibodies to remove human serum albumin (HSA), albumin, IgG, IgA, IgM, IgD, IgE, kappa and lambda light chains, alpha-1-acidglycoprotein, alpha-1-antitrypsin, alpha-2-macroglobulin, apolipoprotein A1, fibrinogen, haptoglobin, and transferrin from serum, plasma, or spinal fluids.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.