原代大鼠皮质神经元 (Sprague Dawley)

Gibco 原代大鼠皮质神经元 (Sprague Dawley) 从第 18 天 Sprague Dawley 大鼠胚胎中分离得到并冻存了解更多信息

货号	池数量
A36512	4 x 10^6

货号 A36512

价格（CNY)

4 x 10^6

Gibco 原代大鼠皮质神经元 (Sprague Dawley) 从第 18 天 Sprague Dawley 大鼠胚胎中分离得到并冻存。这是一种灵活的即用型产品，且是新鲜神经元的优质替代品，每样品瓶中含有 1 百万个细胞/mL。这些神经元解冻后活力可一直保持在 50–80% 范围内，且具有高神经元纯度，且经过功能验证。

•高活力：解冻后活力为 50% 或更高
•高纯度：大于 70% 神经元表型
•功能验证：经过自发性同步钙通量和突触释放活性检测

仅供科研使用。不可用于诊断程序。

产品规格One vial containing 4 million cells

池数量4 x 10^6

产品类型大鼠皮层神经元

数量4 x 10⁶ cells

经过测试细胞纯度、无菌、活性、支原体

细胞类型神经细胞

培养类型贴壁细胞培养

形式Cryopreserved

Unit SizeEach

常见问题解答 (FAQ)

What is the difference between Primary Rat Cortex Neurons, Sprague Dawley and Primary Rat Cortex Neurons?

These neurons differ in the rat strain from which they were isolated: Sprague Dawley vs. Fisher 344. These neurons don't expand anymore. They just grow neurites. The manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0001574-PrimaryRatCortex-HippocampusNeurons-UG.pdf) has instructions for culturing the cells. You can use Neurobasal Plus/B-27 Plus/GlutaMAX to culture them, or just Neurobasal/B-27/GlutaMAX. However, the former combination provides much superior performance than the latter and can maintain neurons longer.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can primary rat neurons be cryopreserved again after they are cultured?

Primary rat neurons cannot be cryopreserved again because they will not revive.

Can primary rat neurons proliferate?

No, primary rat neurons do not proliferate.

View all 原代大鼠皮质神经元 (Sprague Dawley) FAQs