Primary Rat Hippocampal Neurons, Sprague Dawley - FAQs

View additional product information for Primary Rat Hippocampal Neurons, Sprague Dawley - FAQs (A36513)

6 product FAQs found

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the purpose of L-glutamate in the media for primary hippocampal neurons?

Glutamate helps hippocampal neurons sprout and promotes viability. However, L-glutamate can only be supplemented in the medium up to day 4 in culture because it is toxic to mature neurons.

Can primary rat neurons be cryopreserved again after they are cultured?

Primary rat neurons cannot be cryopreserved again because they will not revive.

Can primary rat neurons proliferate?

No, primary rat neurons do not proliferate.

Why does the manual for primary rat neurons have the instruction "Do not expose neurons to air at any time"?

Neurons tend to lift off from the complete medium change, which exposes cells to air. By changing half of the medium, the disturbance can be minimized.