B-27™ Plus 神经元培养系统
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B-27™ Plus 神经元培养系统
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B-27™ Plus 神经元培养系统

B-27 Plus 神经元培养系统是下一代培养基系统,可提供较高的原代啮齿动物来源及人干细胞来源神经元体外生存率。该系统包含 B-27 Plus 补充剂 (50X) 和 Neurobasal了解更多信息
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货号数量
A36534011 System
货号 A3653401
价格(CNY)
3,384.00
Each
添加至购物车
数量:
1 System
价格(CNY)
3,384.00
Each
添加至购物车
B-27 Plus 神经元培养系统是下一代培养基系统,可提供较高的原代啮齿动物来源及人干细胞来源神经元体外生存率。该系统包含 B-27 Plus 补充剂 (50X) 和 Neurobasal Plus 培养基,是引用较多的神经元细胞培养产品 B-27 补充剂和 Neurobasal 培养基进化而来的。它采用优化的配方,升级的生产工艺,以及更严格的原材料和终产品质控手段。与其他神经元细胞培养基系统相比,这些改进实现了较高的神经元细胞存活率,更高的电生理活性,以及更高的功能性成熟度。

B-27 Plus 神经元培养系统可提供:
•原代大鼠神经元比经典 B-27 补充剂和 Neurobasal 培养基至少高 50% 的存活率
• 比其他市售无血清神经元培养基系统更高的原代大鼠、小鼠和人干细胞来源神经元存活率
电生理活性和成熟度更高—相较于 BrainPhys™ 神经元培养基,10 天后原代啮齿动物神经元的自发活性更一致、稳定和同步
加速神经元突起生长并增加突触阳性刻点的数量

B-27 Plus 补充剂可作为含 500 mL 基础培养基的 50X、10 mL 补充剂,无缝替代其他神经元细胞培养系统,如经典的 B-27 补充剂和 Neurobasal 培养基。系统的单个组分也可单独购买。

仅供科研使用。不可用于诊断程序。
规格
细胞类型神经细胞
产品线B-27™、Neurobasal™
产品类型神经元培养系统
数量1 System
有效期12 个月
运输条件环境
分类无血清
培养类型哺乳动物细胞培养
形式液体
血清水平无血清
Unit SizeEach
内容与储存
• 10 mL B-27 Plus 补充剂 (50X),避光储存于 –5 至 –20°C 条件下
• 500 mL Neurobasal Plus 培养基,避光储存于 2–8°C 条件下

常见问题解答 (FAQ)

Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?

We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use the B-27 Plus Neuronal Culture System for electrophysiological studies?

Yes, we have shown improved electrophysiological activity with the B-27 Plus Neuronal Culture System

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I feed my cells with the B-27 Plus Neuronal Culture System?

We recommend performing half medium exchanges every 2-3 days post-plating. Make sure that neurons are not completely exposed to air by not removing all of the medium during exchanges.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.