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View additional product information for KingFisher™ Duo-Ready DNA Ultra 2.0 Prefilled Plates - FAQs (A36584)
10 product FAQs found
Yes. We will be adding protocols for all the sample types that were covered by the original MagMAX DNA Multi-Sample Ultra kits.
We do not recommend adding proteinase K and enhancer solution at the same time in a mastermix because the activity of proteinase K may be reduced. If added together, the total yield of DNA will be low.
The plates and reagents are shipped at room temperature and we recommend storing them at room temperature.
No, KingFisher Duo-Ready DNA Ultra 2.0 and KingFisher Flex-Ready DNA Ultra 2.0 prefilled plates are currently supplied as RUO (Research-Use Only) products.
The KingFisher Duo-Ready DNA Ultra 2.0 and KingFisher Flex-Ready DNA Ultra 2.0 prefilled plates can be used on Presto/Nimbus system, however, optimized scripts must be generated by the end user to accommodate their specific system setups.
Scripts for KingFisher Duo-Ready DNA Ultra 2.0 or KingFisher Flex-Ready DNA Ultra 2.0 prefilled plates can be found on the MagMAX DNA Multi-Sample Ultra 2.0 Kit product page (https://www.thermofisher.com/order/catalog/product/A36570), under Product literature.
We recommend using both Proteinase K and the enhancer solution for all sample types to ensure maximum DNA yields and prevent binding bead stickiness. When either Proteinase K or enhancer solution is eliminated from the workflow, the beads may clump together which in turn will result in bead carry-over into the final eluate and diminished DNA yield.
Note: Proteinase K and enhancer treatment is especially important for whole blood samples.
One exception we have found is with saliva samples. Some saliva collection tubes contain a stabilization solution that allows the sample to be processed without Proteinase K/enhancer solution. Users must test their collection device for optimal conditions.
We recommend purchasing MagMAX Multi-Sample Ultra 2.0 Elution Solution (Cat. No. A36582) to increase the volume in prefilled plates.
Any volume of sample between 50-400 µL can be used in the same run without normalizing sample input volumes or reagent volumes.
The reagents provided in each kit are sufficient for 96 preps ranging from 50-400 µL sample input.