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View additional product information for KingFisher™ Ready DNA Ultra 2.0 Prefilled Plates, for KingFisher Flex instruments - FAQs (A36586)
32 product FAQs found
Yes. We will be adding protocols for all the sample types that were covered by the original MagMAX DNA Multi-Sample Ultra kits.
We do not recommend adding proteinase K and enhancer solution at the same time in a mastermix because the activity of proteinase K may be reduced. If added together, the total yield of DNA will be low.
The plates and reagents are shipped at room temperature and we recommend storing them at room temperature.
No, KingFisher Duo-Ready DNA Ultra 2.0 and KingFisher Flex-Ready DNA Ultra 2.0 prefilled plates are currently supplied as RUO (Research-Use Only) products.
The KingFisher Duo-Ready DNA Ultra 2.0 and KingFisher Flex-Ready DNA Ultra 2.0 prefilled plates can be used on Presto/Nimbus system, however, optimized scripts must be generated by the end user to accommodate their specific system setups.
Scripts for KingFisher Duo-Ready DNA Ultra 2.0 or KingFisher Flex-Ready DNA Ultra 2.0 prefilled plates can be found on the MagMAX DNA Multi-Sample Ultra 2.0 Kit product page (https://www.thermofisher.com/order/catalog/product/A36570), under Product literature.
We recommend using both Proteinase K and the enhancer solution for all sample types to ensure maximum DNA yields and prevent binding bead stickiness. When either Proteinase K or enhancer solution is eliminated from the workflow, the beads may clump together which in turn will result in bead carry-over into the final eluate and diminished DNA yield.
Note: Proteinase K and enhancer treatment is especially important for whole blood samples.
One exception we have found is with saliva samples. Some saliva collection tubes contain a stabilization solution that allows the sample to be processed without Proteinase K/enhancer solution. Users must test their collection device for optimal conditions.
We recommend purchasing MagMAX Multi-Sample Ultra 2.0 Elution Solution (Cat. No. A36582) to increase the volume in prefilled plates.
Any volume of sample between 50-400 µL can be used in the same run without normalizing sample input volumes or reagent volumes.
The reagents provided in each kit are sufficient for 96 preps ranging from 50-400 µL sample input.
No, the processing volume refers to the maximum volume that can be held in the 24 Deep Well plates. This incorporates the volume of binding buffers and beads, so when scaling up reaction volumes, it is important to make sure that the total volume does not exceed 5 mL.
No, once a protocol was stopped, it will restart from the beginning. The extraction procedure can either be finished manually by using a magnetic stand, or a modified version of the original KingFisher Flex script would have to be created that starts off at the point where the run was stopped.
Set up the protocol on the BindIt software. After the first 8 plates are done, the instrument will pause and instruct you to switch out the plates.
The transport locks are only necessary when relocating the instrument.
We strongly recommend that you maintain the specified volumes within the defined limits to avoid spillover and to help ensure the best performance at the most efficient level.
This happens sometimes but it will not affect the yield because the sample has been released from the particles.
If the starting material is too viscous, the magnetic rods will not be able to collect the particles. Dilute the sample and check that the sample is properly homogenized/lysed. Adding low amounts of detergent will improve the collection of magnetic particles as well. Quickly centrifuge the plate to sediment the particles to the bottom of the plate.
The protocol will not start without the tip combs inserted into the tip comb holder.
Wipe the magnetic rods with a soft cloth or tissue paper soaked in a mild detergent solution, soap solution, or alcohol.
The heating block warms up at the rate of about 10°C per minute, so it will take about 6 minutes.
Both deep-well plates and KingFisher 96 plates can be used during the same run. Therefore, it is possible to start the processing by using larger volumes (in a deep-well plate) and elute the purified sample to a smaller volume (in a KingFisher 96 or MagMAX 96 standard plate).
This can be done by running the Change magnet protocol under the Maintenance menu using the “up” and “down” cursor keys.
The KingFisher Flex magnets are made of material that is very stable. The magnetic field will not get weaker. However, extreme mechanical force or heating can cause damage to the magnets.
Caution: It is very important to keep the KingFisher Flex heads away from each other and from other magnets at all times. Clashing together of the magnets may cause serious damage to the magnets.
KingFisher Flex uses a USB port, whereas the MagMAX Express- 96 requires a RS-232 port.
No. Protocols cannot be transferred back from the instrument to the computer. Protocols need to be previously stored in the BindIt or KingFisher software to make edits.
No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.
For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.
No, we strongly recommend that you use the MagMAX plates or KingFisher plates since they were specifically designed to be used with KingFisher Flex tip combs to attain maximal performance. Plates from other manufacturers may not be compatible with the KingFisher Flex heating blocks. They may also cause unexpected problems, such as cross-contamination, due to the divergent well volume and bottom height of the plate.
There are 4 magnetic head options available for the KingFisher Flex Magnetic Particle Processor:
- KingFisher Flex head for MagMAX Express-96 Deep Well plate
- KingFisher Flex head for KingFisher 24 Deep Well plate
- KingFisher Flex head for KingFisher 96 plate
- KingFisher Flex head for PCR plate, skirted
Yes, all MagMAX kits can be used with the KingFisher Flex Magnetic Particle Processor.