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View additional product information for Oncomine™ Myeloid Research Assay - FAQs (A36940, A51770)
11 product FAQs found
We have validated the use of the MagMAX Total Nucleic Acid Isolation Kit (Cat. No. AM1840), which is compatible with whole blood and bone marrow samples. We recommend using your preferred commercially available kit to sequentially isolate high quality gDNA and RNA from research samples for use in library preparation.
10 ng of gDNA per pool at a minimum concentration of ≥1.48 ng/µL for manual library preparation 10 ng of RNA at a minimum concentration of ≥2.5 ng/µL for manual library preparation
Blood and bone marrow samples are compatible with the Oncomine Myeloid Research Assay. This kit is not intended for use with FFPE samples.
The kit has been validated on the Ion GeneStudio S5 Series Systems and Ion PGM System.
With the Ion GeneStudio S5 Series Systems, we recommend using:
-Ion 510 & Ion 520 & Ion 530 Kit - Chef (Cat. No. A34019)
-Ion 530 Chip Kit (Cat. No. A27764).
With the Ion PGM System, we recommend using:
-Ion PGM Hi‑Q View OT2 Kit (Cat. No. A29900)
-Ion PGM Hi‑Q View Chef 400 Kit (Cat. No. A30798)
-Ion PGM Hi‑Q View Sequencing Kit (Cat. No. A30044)
-Ion 318 Chip v2 BC (Cat. No. 4488150)
Note: Ion 540 Chip is NOT compatible with the Oncomine Myeloid Research Assay as 400 base pair (850 Flows) sequencing is required.
The assay can be run on both the Ion PGM System and Ion S5/S5XL systems.
-PGM Plexy = 4 samples per Ion 318 Chip
-S5 Manual Library Prep Plexy = 12 samples per Ion 530 Chip
-S5 Chef Library Prep Plexy = 8 samples per Ion 530 Chip
The recommended controls are:
-AcroMetrix Oncology Hotspot Control, Cat. No. 969056 (https://www.thermofisher.com/order/catalog/product/969056)
-Seraseq Myeloid Fusion RNA Mix, Cat. No. 0710-0407 (https://www.seracare.com/Seraseq-Myeloid-Fusion-RNA-Mix-0710-0407/)
-Seraseq Myeloid Mutation DNA Mix, Cat. No. 0710-0408 (https://www.seracare.com/Seraseq-Myeloid-Mutation-DNA-Mix-0710-0408/)
For automated library preparation using the Ion Chef System, we offer the Oncomine Myeloid Research Assay - Chef-Ready (Cat. No. A36941).
The Oncomine Myeloid Research Assay (Cat. No. A36940) consists of the Oncomine Myeloid DNA Panel (2-pool), Oncomine Myeloid RNA Panel (single pool), and two Ion AmpliSeq Library Kit Plus kits. This kit is used to create the library manually. Sufficient reagents are provided for the rapid preparation of 24 sample libraries (barcoded DNA- and RNA-libraries). Each sample will have 3 libraries (2 DNA and 1 RNA).
There are 3 primer pools, 2 DNA pools, and 1 RNA pool.
The panel is comprised of 40 key DNA genes and a broad fusion panel of 29 driver genes to cover the most relevant targets in major myeloid disorders such as acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML).
The Oncomine Myeloid Research Assay is a comprehensive, targeted NGS assay designed to assist in the understanding of myeloid cancer. Specifically, it interrogates all relevant DNA mutations and fusion transcripts associated with myeloid disorders in a single NGS run. The Oncomine Myeloid Research Assay, Cat. No. A36940, is for manual library preparation and Oncomine Myeloid Research Assay-Chef Ready, Cat. No. A36941, is for automated library preparation using the Ion Chef System.