Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands.
AuthorsLi J, Shang G, Chen YJ, Brautigam CA, Liou J, Zhang X, Bai XC
JournalElife
PubMed ID31535977
'RET is a receptor tyrosine kinase (RTK) that plays essential roles in development and has been implicated in several human diseases. Different from most of RTKs, RET requires not only its cognate ligands but also co-receptors for activation, the mechanisms of which remain unclear due to lack of high-resolution structures ... More
Intracerebroventricular Delivery of Recombinant NAMPT Deters Inflammation and Protects Against Cerebral Ischemia.
AuthorsChen F, Weng Z, Xia Q, Cao C, Leak RK, Han L, Xiao J, Graham SH, Cao G
JournalTransl Stroke Res
PubMed ID30820847
'Our previous study indicated that nicotinamide phosphoribosyltransferase (NAMPT) is released from cells and might be an important extracellular neuroprotective factor in brain ischemia. Here, we tested whether NAMPT protects against ischemic brain injury when administered directly into the intracerebroventricular (ICV) compartment of the cranium. Recombinant NAMPT protein (2 µg) was delivered ... More
AuthorsYoshida S, Miyagawa S, Toyofuku T, Fukushima S, Kawamura T, Kawamura A, Kashiyama N, Nakamura Y, Toda K, Sawa Y
JournalSci Rep
PubMed ID32165680
'Avoiding immune rejection after allogeneic induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) transplantation is a concern. However, mesenchymal stem cells (MSCs) can suppress immune rejection. To determine whether MSC co-transplantation can reduce immune rejection after allogeneic iPSC-CM transplantation, the latter cell type, harbouring a luciferase transgene, was subcutaneously transplanted alone or ... More
Exosome-based delivery of super-repressor I?Ba relieves sepsis-associated organ damage and mortality.
AuthorsChoi H, Kim Y, Mirzaaghasi A, Heo J, Kim YN, Shin JH, Kim S, Kim NH, Cho ES, In Yook J, Yoo TH, Song E, Kim P, Shin EC, Chung K, Choi K, Choi C
JournalSci Adv
PubMed ID32285005
As extracellular vesicles that play an active role in intercellular communication by transferring cellular materials to recipient cells, exosomes offer great potential as a natural therapeutic drug delivery vehicle. The inflammatory responses in various disease models can be attenuated through introduction of super-repressor I?B (srI?B), which is the dominant active ... More
Improved cutaneous genetic immunization by microneedle array delivery of an adjuvanted adenovirus vaccine.
AuthorsErdos G, Balmert SC, Carey CD, Falo GD, Patel NA, Zhang J, Gambotto A, Korkmaz E, Falo LD
JournalJ Invest Dermatol
PubMed ID32330464
Fibrin fragment E potentiates TGF-ß-induced myofibroblast activation and recruitment.
AuthorsFuchs PÖ, Calitz C, Pavlovic N, Binet F, Solbak SMØ, Danielson UH, Kreuger J, Heindryckx F, Gerwins P
JournalCell Signal
PubMed ID32334027
Fibrin is an essential constituent of the coagulation cascade, and the formation of hemostatic fibrin clots is central to wound healing. Fibrin clots are over time degraded into fibrin degradation products as the injured tissue is replaced by granulation tissue. Our goal was to study the role of the fibrin ... More