A compact STED microscope providing 3D nanoscale resolution.
AuthorsWildanger D, Medda R, Kastrup L, Hell SW,
JournalJ Microsc
PubMed ID19772534
'The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based resolution down to (19 +/- 3) nm in the focal ... More
Quencher-free multiplexed monitoring of DNA reaction circuits.
AuthorsPadirac A, Fujii T, Rondelez Y,
JournalNucleic Acids Res
PubMed ID22753028
We present a simple yet efficient technique to monitor the dynamics of DNA-based reaction circuits. This technique relies on the labeling of DNA oligonucleotides with a single fluorescent modification. In this quencher-free setup, the signal is modulated by the interaction of the 3'-terminus fluorophore with the nucleobases themselves. Depending on ... More