Ion AmpliSeq™ HD Dual Barcode Kit 1-24 - FAQs

We have taken the 96-well plate in the Ion AmpliSeq HD Dual Barcode Kit 1-24 through several freeze-thaw cycles with little to no reduction in performance.
Note: Sealing the plate between uses is key when using the plate for multiple experiments.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Ion AmpliSeq HD Dual Barcode Kit 1-24 is compatible with Torrent Suite Software version 5.10 and above.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The kit is shipped at room temperature, but should be stored at -20 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Ion AmpliSeq HD Dual Barcode Kit 1-24 is good for 24 samples. Each barcode is good for one sample.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

No, it is only compatible with manual sample preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Ion AmpliSeq HD Dual Barcode Kit 1-24 provides a set of 24 unique dual-barcode primers designed and validated for optimal performance with Ion GeneStudio S5 series sequencers. The kit enables up to 24 samples to be multiplexed on a single chip and improves the accuracy of multiplex sequencing by reducing sample mis-assignment. The barcode kit is only compatible with the Ion AmpliSeq HD Library Kit (Cat. No. A37694) and should not be used with any other library kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.