Tandem Mass Tag (TMT) 试剂专门设计用于通过串联质谱分析对不同样品中的蛋白进行鉴别和定量测定。TMT11-131C 标签结构与其他 TMT10pex 标签相同,但 TMT11-131C 质量报告子仅使用 13C 同位素标记。添加 TMT11-131C 标签可以将原始 TMT10plex 试剂的 131 质量报告离子分离为 N 和 C 变体,使用高分辨率 Orbitrap MS 仪器创建第 11 通道进行相对定量测定。
Cell-type and subcellular compartment-specific APEX2 proximity labeling reveals activity-dependent nuclear proteome dynamics in the striatum.
Authors:Dumrongprechachan V,Salisbury RB,Soto G,Kumar M,MacDonald ML,Kozorovitskiy Y
Journal:Nature communications
PubMed ID:34381044
The vertebrate brain consists of diverse neuronal types, classified by distinct anatomy and function, along with divergent transcriptomes and proteomes. Defining the cell-type specific neuroproteomes is important for understanding the development and functional organization of neural circuits. This task remains challenging in complex tissue, due to suboptimal protein isolation techniques ... More
Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells.
Authors:Boussardon C,Carrie C,Keech O
Journal:Journal of experimental botany
PubMed ID:37026385
Plastids are complex organelles that vary in size and function depending on the cell type. Accordingly, they can be referred to as amyloplasts, chloroplasts, chromoplasts, etioplasts, or proplasts, to only cite a few. Over the past decades, methods based on density gradients and differential centrifugation have been extensively used for ... More
Correction: Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies
Authors:Armstrong LA, Lange SM, Cesare V, Matthews SP, Nirujogi RS, Cole I, Hope A, Cunningham F, Toth R, Mukherjee R, Bojkova D, Gruber F, Gray D, Wyatt PG, Cinatl J, Dikic I, Davies P, Kulathu Y.
Decellularization alters the unfavorable regenerative adverse microenvironment of the injured spinal cord to support neurite outgrowth.
Authors:Hu J,Shangguan J,Askar P,Xu J,Sun H,Zhou S,Zhu C,Su W,Gu Y
Journal:Annals of translational medicine
PubMed ID:36172103
BACKGROUND: Acellular tissue has been transplanted into the injury site as an external microenvironment to intervene with imbalance microenvironment that occurs after spinal cord injury (SCI) and stimulating axonal regeneration, although the mechanism is unclear. Given decellularization is the key means to obtain acellular tissues, we speculated changes in the ... More