Pro-Detect™ 快速 DYKDDDDK-His 检测试剂盒
Pro-Detect™ 快速 DYKDDDDK-His 检测试剂盒
Thermo Scientific™

Pro-Detect™ 快速 DYKDDDDK-His 检测试剂盒

Thermo Scientific Pro-Detect 快速 DYKDDDDK-His 检测试剂盒是单次使用、测深尺侧向层析试剂盒,用于快速、轻松地检测蛋白表达和纯化系统中的 DYKDDDDK-His 双标签蛋白了解更多信息
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货号数量
A3850010 次检测
货号 A38500
价格(CNY)
819.00
飞享价
Ends: 31-Dec-2025
1,292.00
共减 473.00 (37%)
Each
添加至购物车
数量:
10 次检测
价格(CNY)
819.00
飞享价
Ends: 31-Dec-2025
1,292.00
共减 473.00 (37%)
Each
添加至购物车
Thermo Scientific Pro-Detect 快速 DYKDDDDK-His 检测试剂盒是单次使用、测深尺侧向层析试剂盒,用于快速、轻松地检测蛋白表达和纯化系统中的 DYKDDDDK-His 双标签蛋白。该侧向层析检测使用基于膜的试纸条,该试纸条包被 DYKDDDDK-His 标签特异性的染料结合偶联抗体,可在十分钟内提供可视化有色读数以供检测。该测定可用于直接从细胞培养基(上清液)或细胞裂解物中检测 DYKDDDDK-His 标签蛋白,无需使用任何特殊仪器或进行处理。

Pro-Detect 快速检测试剂盒的特点:
易于采用—侧向层析试纸条可以在表达早期和整个表达过程中使用以监测和确认所需蛋白标记靶标的表达
简单、快速的方法—使用提供的稀释液稀释样品并将其浸入侧向层析试纸条中,可在 5-10 分钟内获得可视化结果
灵敏—在上清液或细胞裂解物样品中检测到浓度低至 μg/mL 浓度的蛋白标记
特异性—该测定不与常见裂解和蛋白制备试剂发生交叉反应
可靠—结果与其他抗体免疫检测(包括 Western 印迹和 ELISA)相似
有效期—在 4°C 下可稳定保存至少一年

仅需将适当稀释的组织培养物上清液、裂解物或小纯化蛋白样品添加至试管或微孔板的孔中并浸入侧向层析试纸条,进行每次 Pro-Detect 快速测定。包埋在试纸条的金偶联检测抗体与样品中的标签蛋白形成复合物。这些复合物穿过条带膜的长度并在检测线和对照线处结合。结果显示为红色条带。
仅供科研使用。不可用于诊断程序。
规格
检测侧向层析快速检测
可检测分析物DYKDDDDK-His (FLAG-His) 双标签蛋白
适用于(应用)侧向层析快速检测
产品线Pro-Detect™
产品类型检测
数量10 次检测
运输条件湿冰
特异性蛋白标记
足够用于10 次检测
靶标Protein
检测方法显色
Unit SizeEach
内容与储存
• 10 条快速检测试纸条
• 样品稀释液,15 mL

足以进行 10 次试验测定

在 2–8°C 下储存。

常见问题解答 (FAQ)

Can I use the Pro-Detect assay kits for rapidly isotyping antibodies?

The Pro-Detect Rapid Antibody Isotyping Kit is a lateral-flow assay with high sensitivity for rapid determination of the immunoglobulin class, subclass, and light chain type of monoclonal antibodies. Two different formats of rapid isotyping kits are available. These include dipstick format and cassette-based format. One can use these kits to identify the isotype of a mouse, rat, or human antibody. Make sure to follow the recommended dilution guidelines.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am using a Pro-Detect assay kit and cannot differentiate banding pattern between control lysates and lysates that are actually expressing proteins of interest. What is the problem?

1) It is likely that there could be a substance interfering with the assay. Check the compatibility table on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html), under “Detergent and salt compatibility limits”.
2) The expression level of the actual tagged protein is below the detectable range of the assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Using Pro-Detect assay kits, can I detect the protein of interest in a mixture of proteins such as a crude lysate?

Yes, first, make sure that the protein of interest is tagged and that we offer a Pro-Detect assay kit that can detect that tag. Secondly, make sure that you follow the guidelines to detect proteins in crude lysate and if necessary increase or decrease the lysate concentration to bring the protein of interest within the detectable range of the assay.

Note that the guidelines for the recommended detection ranges reflect the concentration of the pure tagged protein in a given sample mixture.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do I interpret the gradual disappearance of four lines in the Pro-Detect competition assay kits?

As the concentration of the tag in the protein sample increases, the number of lines will decrease until they all disappear. The concentration of the tagged proteins is inversely related to the number of lines appearing on the strip.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are the results of Pro-Detect assay kits comparable to western blotting?

Yes. Western blotting is a widely used and accepted tool to identify a specific protein in a complex mixture. However, it is a time-consuming and labor-intensive process, with many preparative steps and time intervals to manage. The Pro-Detect rapid assays are able to provide comparable accuracy but with much shorter processing time (10-15 min) and less hands-on steps. For further information, please see the data shown on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html) under “Results are comparable to western blotting”.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.