The Thermo Scientific Pro-Detect Rapid MBP Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of maltose binding protein (MBP)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the MBP tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect MBP-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid assay kits:
• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low μg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.