Thermo Scientific™

Pro-Detect™ Rapid V5 Competitive Assay Kit

货号: A38513
Thermo Scientific™

Pro-Detect™ Rapid V5 Competitive Assay Kit

货号: A38513
货号
A38513
单位规格
10 assays
价格(CNY)
价格 1,227.00
飞享价 803.00
(结束31-Dec-2024)
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货号单位规格价格(CNY)库存状况数量
A3851310 assays
价格 1,227.00
飞享价 803.00
(结束31-Dec-2024)
您的价格
***
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The Thermo Scientific Pro-Detect Rapid V5 Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of V5-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to V5 tag to provide a qualitative detection within ten minutes. This assay can be used to detect V5-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid competitive assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low μg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.
For Research Use Only. Not for use in diagnostic procedures.

规格

可检测分析物
V5 标签蛋白
检测
侧向层析快速检测
检测方法
发色
产品线
Pro-Detect™
产品类型
检测
特异性
蛋白标记
足够用于
10 次检测
适用于(应用)
侧向层析快速检测
数量
10 次检测
运输条件
湿冰
靶标
Protein

内容与储存

• 10 个快速检测试纸条
• 样品稀释液,15 mL

足以进行 10 次检测测定

储存于 2–8°C。

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