Collibri™ Library Quantification Kit, 500 reactions - FAQs

查看更多产品信息 Collibri™ Library Quantification Kit - FAQs (A38524100, A38524500)

4 个常见问题解答

My qPCR instrument requires ROX. Which ROX dye do you recommend using with the Collibri Library Quantification Kit?

The Collibri Library Quantification Kit contains the Collibri Library Quantification Master Mix that already contains a universal concentration of ROX passive reference dye, so there is no need for additional adjustment with ROX dye regardless of the instrument you are working with.

Do I need to dilute the library prior to qPCR using the Collibri Library Quantification Kit?

Yes, the prepared library has to be diluted prior to running qPCR. Generally, it is recommended to run two dilution points, 1:10000 and 1:100000. For an established workflow where approximate library quantity is known, dilutions can be adjusted, but they need to fall within the range of the Collibri DNA Standards. Note that libraries should be diluted in the Collibri Library Dilution buffer that is included in the Collibri Library Quantification Kit. Dilution of the library stabilizes library molecules at low concentrations, producing accurate and reproducible results.

With the Collibri Library Quantification Kit, what effect do the visual cues for mixing have on library quantification results?

Colors in the Collibri Library Quantification Kit components have no effect on the universal passive reference dye, qPCR performance, or quantification results.

Can Collibri Library Quantification Kit be used with NGS libraries prepared for Ion Torrent sequencing?

No. However, the Collibri Library Quantification Kit contains the Collibri Library Quantification Master Mix that has premixed primers which are compatible with P5 and P7 adaptor sequences specific to the Illumina platform.