Are PCR products generated using the Collibri Library Amplification Master Mix blunt-ended?
Yes, PCR products generated using the Collibri Library Amplification Master Mix are blunt-ended.
Is Collibri Library Amplification Master Mix compatible with magnetic beads?
Yes, Collibri Library Amplification Master Mix is compatible with magnetic beads provided in Collibri library preparation kits and in Agencourt AmPure XP system from Beckman Coulter.
What is the fidelity of the Collibri Library Amplification Master Mix?
The Collibri Library Amplification Master Mix contains Platinum SuperFi DNA Polymerase that has strong 3' to 5' exonuclease (proofreading) activity for superior amplification accuracy, greater than 100-fold in comparison with Taq polymerase.
What are the recommended storage conditions for the Collibri Library Amplification Master Mix?
We recommend storing the Collibri Library Amplification Master Mix at -20 degrees C.
Is Collibri Library Amplification Master Mix suitable for amplification of libraries prepared from FFPE samples?
Yes, however, additional PCR cycles (1-3) are typically needed with FFPE DNA or other challenging samples. To determine the optimal PCR conditions, use the table in the user manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017582_Platinum_SuperFi_LibraryAmp_MM_w_Primer_Mix_UG.pdf) with the required number of PCR cycles per sample size as a starting point.