具有专利技术的反应缓冲液含有所有必要的 PCR 成分和添加剂,无论 GC 含量如何,均能实现高效、均一的文库扩增,有助于提高富含 GC 和 AT 的序列及其他复杂区域的覆盖度。高扩增效率有助于使用更少的循环获得理想的产量。
Platinum 热启动技术在室温下可抑制 DNA 聚合酶活性,实现室温反应构建并使预配制 PCR 反应体系在 PCR 前可储存最长 24 小时。
仅供科研使用。不可用于诊断程序。
规格
保真度(相对于 Taq)≥100
适用于(应用)测序
形式Liquid
高 GC PCR 扩增效果高
热启动内置热启动
反应次数250
突出端平末端
产品线Collibri™
产品类型文库扩增预混液
数量250 reactions
反应形式SuperMix 或预混液
样品类型DNA, RNA
运输条件干冰
测试时间标准
Unit SizeEach
内容与储存
• 5 x 1.25 mL Collibri 文库扩增预混液 (2X) • 5 x 500 µL 引物混合物
储存在 -20°C 下。
常见问题解答 (FAQ)
Are PCR products generated using the Collibri Library Amplification Master Mix blunt-ended?
Yes, PCR products generated using the Collibri Library Amplification Master Mix are blunt-ended.
Is Collibri Library Amplification Master Mix compatible with magnetic beads?
Yes, Collibri Library Amplification Master Mix is compatible with magnetic beads provided in Collibri library preparation kits and in Agencourt AmPure XP system from Beckman Coulter.
What is the fidelity of the Collibri Library Amplification Master Mix?
The Collibri Library Amplification Master Mix contains Platinum SuperFi DNA Polymerase that has strong 3' to 5' exonuclease (proofreading) activity for superior amplification accuracy, greater than 100-fold in comparison with Taq polymerase.
What are the recommended storage conditions for the Collibri Library Amplification Master Mix?
We recommend storing the Collibri Library Amplification Master Mix at -20 degrees C.
Is Collibri Library Amplification Master Mix suitable for amplification of libraries prepared from FFPE samples?
Yes, however, additional PCR cycles (1-3) are typically needed with FFPE DNA or other challenging samples. To determine the optimal PCR conditions, use the table in the user manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017582_Platinum_SuperFi_LibraryAmp_MM_w_Primer_Mix_UG.pdf) with the required number of PCR cycles per sample size as a starting point.