Pro-Detect™ 快速抗体同种型检测试剂盒(小鼠)
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Pro-Detect™ 快速抗体同种型检测试剂盒(小鼠)
Thermo Scientific™

Pro-Detect™ 快速抗体同种型检测试剂盒(小鼠)

Thermo Scientific Pro-Detect 快速抗体同种型检测试剂盒 - 小鼠是单次使用、测深尺侧向层析试剂盒,用于快速、轻松地测定小鼠单克隆抗体类别和亚类鉴别。该抗体分型测定使用包被有染料结合偶联抗体的基于膜的试纸条,可在十分钟内提供单克隆抗体同种型的可视化有色读数。该试剂盒可测定小鼠了解更多信息
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货号数量
A3855010 次测试
货号 A38550
价格(CNY)
2,046.00
飞享价
Ends: 31-Dec-2025
3,227.00
共减 1,181.00 (37%)
Each
添加至购物车
数量:
10 次测试
价格(CNY)
2,046.00
飞享价
Ends: 31-Dec-2025
3,227.00
共减 1,181.00 (37%)
Each
添加至购物车
Thermo Scientific Pro-Detect 快速抗体同种型检测试剂盒 - 小鼠是单次使用、测深尺侧向层析试剂盒,用于快速、轻松地测定小鼠单克隆抗体类别和亚类鉴别。该抗体分型测定使用包被有染料结合偶联抗体的基于膜的试纸条,可在十分钟内提供单克隆抗体同种型的可视化有色读数。该试剂盒可测定小鼠 IgG1、IgG2a、IgG2b、IgG3、IgA、IgM 以及轻链鉴别(κ 轻链相对于 λ 轻链)。

Pro-Detect 快速抗体分型检测试剂盒的特点:
有效期长—在 4°C 下可稳定至少一年
简单工作流程—使用提供的稀释液稀释抗体样品,并浸入侧向层析试纸条
快速—在 5–10 分钟内出现表明抗体同种型的条带
灵敏—可检测浓度低至 ng/mL 的组织培养基或腹水中的抗体
特异性—该测定不与胎牛血清 (FBS) 发生交叉反应
可靠—结果与基于标准 ELISA 的分型试验相似

仅需将适当稀释的组织培养物上清液或小鼠腹水样品添加至试管或微孔板的孔中并浸入侧向层析试纸条,进行每次 Pro-Detect 快速抗体分型测定。包埋在试纸条膜的金偶联物与样品中的抗体形成特定类型和亚类可溶复合物。这些复合物在穿过膜的长度,在膜的抗同种型和特定类型抗体浸渍区域溶解。结果显示为打印类别或亚类描述上方的红色条带,指示存在抗体同种型。

确定单克隆抗体的类别和亚类对于计划最佳的免疫球蛋白纯化方法非常有用。例如,小鼠 IgA 和 IgM 最适合按大小纯化(即凝胶排阻)或使用免疫亲和分离柱纯化。小鼠 IgG2a 和 IgG2b 在 pH 值 7–8 下使用固定化蛋白 A 进行纯化,而小鼠 IgG1 在 pH 值 8–9 下与蛋白 A 的结合性最佳。包含 κ 轻链的免疫球蛋白可使用固定化蛋白 L 进行纯化。

Pro-Detect 快速抗体分型检测试剂盒与组织培养物上清液和小鼠腹水液体兼容,且比传统基于乳胶-微珠的测深尺测定更灵敏,比基于 ELISA 的分型测定快得多。该试剂盒仅需要 0.5 μL 腹水液体、2–22 μL 细胞培养物上清液或 1.5 ng 纯化抗体。
仅供科研使用。不可用于诊断程序。
规格
检测同种型测定
可检测分析物IgG1、IgG2a、IgG2b、IgG3、IgA、IgM;κ 和 λ 轻链
适用于(应用)侧向层析快速检测
产品线Pro-Detect™
产品类型检测
数量10 次测试
运输条件湿冰
特异性抗体
足够用于10 个反应
检测方法显色
样品类型细胞培养基, 腹水
目标种属小鼠
Unit SizeEach
内容与储存
• 10 条快速同种型检测试纸条 1
• 10 条快速抗体同种型检测试纸条 2
• 样品稀释液,4 mL

足够进行 10 同种型测定

在 2–8°C 下储存。

常见问题解答 (FAQ)

Can I use the Pro-Detect assay kits for rapidly isotyping antibodies?

The Pro-Detect Rapid Antibody Isotyping Kit is a lateral-flow assay with high sensitivity for rapid determination of the immunoglobulin class, subclass, and light chain type of monoclonal antibodies. Two different formats of rapid isotyping kits are available. These include dipstick format and cassette-based format. One can use these kits to identify the isotype of a mouse, rat, or human antibody. Make sure to follow the recommended dilution guidelines.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am using a Pro-Detect assay kit and cannot differentiate banding pattern between control lysates and lysates that are actually expressing proteins of interest. What is the problem?

1) It is likely that there could be a substance interfering with the assay. Check the compatibility table on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html), under “Detergent and salt compatibility limits”.
2) The expression level of the actual tagged protein is below the detectable range of the assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Using Pro-Detect assay kits, can I detect the protein of interest in a mixture of proteins such as a crude lysate?

Yes, first, make sure that the protein of interest is tagged and that we offer a Pro-Detect assay kit that can detect that tag. Secondly, make sure that you follow the guidelines to detect proteins in crude lysate and if necessary increase or decrease the lysate concentration to bring the protein of interest within the detectable range of the assay.

Note that the guidelines for the recommended detection ranges reflect the concentration of the pure tagged protein in a given sample mixture.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How do I interpret the gradual disappearance of four lines in the Pro-Detect competition assay kits?

As the concentration of the tag in the protein sample increases, the number of lines will decrease until they all disappear. The concentration of the tagged proteins is inversely related to the number of lines appearing on the strip.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are the results of Pro-Detect assay kits comparable to western blotting?

Yes. Western blotting is a widely used and accepted tool to identify a specific protein in a complex mixture. However, it is a time-consuming and labor-intensive process, with many preparative steps and time intervals to manage. The Pro-Detect rapid assays are able to provide comparable accuracy but with much shorter processing time (10-15 min) and less hands-on steps. For further information, please see the data shown on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html) under “Results are comparable to western blotting”.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.