MagMAX™ Saliva gDNA Isolation Kit, 500 Reactions - FAQs

View additional product information for MagMAX™ Saliva gDNA Isolation Kit - FAQs (A39060, A39059)

10 product FAQs found

The MagMAX Saliva gDNA Lysis/Binding Solution and Wash I Solution have precipitates in them. Can I still use the buffers?

At lower temperatures, the components of the Lysis/Binding Solution and Wash I Solution may precipitate. To get rid of the precipitate, just warm the solutions to room temperature and invert bottles gently to solubilize the reagents before use.

Can I process less than 200 µL of saliva with the MagMAX Saliva gDNA Isolation Kit?

Yes you can, but the gDNA yields will be very low.

Will the MagMAX Saliva gDNA Isolation Kit work for sample types besides saliva?

No, this kit is specialized for freshly collected saliva and stabilized saliva samples only.

Do you sell the buffers and beads included in the MagMAX Saliva gDNA Isolation Kit as standalone items?

Yes, we offer the following kit components as stand-alone items to accommodate custom workflows or reagent volumes that have been optimized for your specific automation needs:
MagMAX Saliva gDNA Binding Beads: Cat. No. A39061
MagMAX Saliva gDNA Wash I Solution: Cat. No. A39062
MagMAX Saliva gDNA Lysis/Binding Solution: Cat. No. A39063
MagMAX Saliva gDNA Elution Solution: Cat. No. A39064

What is the formulation of the MagMAX Saliva gDNA Elution Solution?

Unfortunately, we cannot give out the exact formulation, but it is similar to low TE (10 mM Tris base, 0.1 mM EDTA) and is compatible with microarray, PCR, and NGS downstream applications.

Can I use Tris-HCl or water to elute DNA from the MagMAX Saliva gDNA Binding Beads?

This is not recommended. Switching the elution solution will impact the maximum yield and may impact binding bead carry-over into the eluate.

With the MagMAX Saliva gDNA Isolation Kit, do I use different protocols for processing fresh and stabilized saliva?

No, the same protocols are used for fresh and stabilized saliva.

Do you offer the Wash II Solution from the MagMAX Saliva gDNA Isolation Kit as a standalone item?

Wash II Solution is 80% ethanol and we do not offer it as a stand-alone item. It must be purchased separately from a different vendor.

How much saliva sample can I use with the MagMAX Saliva gDNA Isolation Kit?

You can use 50-500 µL of saliva in a 96-well plate. We also have protocols for processing 1 mL and 2 mL of saliva in a 24-well plate.

Saliva samples can be processed with the MagMAX DNA Multi-Sample Ultra 2.0 Kit as well as MagMAX Saliva gDNA Isolation Kit. What is the difference between these kits and which one should I use?

Both kits use different magnetic beads and different buffers. Both kits can be used with stabilized and fresh saliva samples, but if you are exclusively working with saliva samples, we recommend using the MagMAX Saliva gDNA Isolation Kit. This kit has a faster and more optimized workflow for isolating genomic DNA from saliva samples.