L-selenomethionine is a known cell toxin. Therefore, it is important to grow cells in the present of this amino acid for the shortest amount of time required to achieve maximal labeling. We recommend adding L-selenomethionine to the culture just prior to transfection (in Expi293 Met (-) Expression Medium). You may also starve cells for 6 hr by culturing cells in Expi293 Met (-) Expression Medium (without supplementation) and then proceed with L-selenomethionine add-back and transfection.
Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.