Expi293™ GnTI 表达系统试剂盒

Expi293 GnTI 表达系统试剂盒是 Expi293 平台的一部分，用于哺乳动物 293 细胞的快速、高产量瞬时蛋白表达。系统试剂盒以设计的 Expi293F了解更多信息

货号	数量
A39250	1 kit

货号 A39250

价格（CNY)

1 kit

Expi293 GnTI 表达系统试剂盒是 Expi293 平台的一部分，用于哺乳动物 293 细胞的快速、高产量瞬时蛋白表达。系统试剂盒以设计的 Expi293F 细胞为中心，这些细胞无 N-乙酰葡糖胺基转移酶 I (GNTI) 活性，因此缺乏复合物 N-聚糖。通过选择性敲除亲代 Expi293F 细胞中的 GnTI 基因建立 Expi293F GnTI 细胞系。该细胞系是均相糖基化重组蛋白高产表达的有力工具。

Expi293 GnTI 表达系统试剂盒的特点包括：
•表达蛋白的均相 N-糖基化
•与亲代 Expi293F 细胞的蛋白产量等效—高达 1 g/L
•蛋白产量显著高于其他 HEK293 GnTI 细胞系替代品
•在 Expi293 表达培养基中悬浮、高密度培养 Expi293F GnTI 细胞
•利用 ExpiFectamine 293 试剂联合专用转染增强剂进行高效瞬时转染

Expi293 GnTI 表达系统试剂盒包括的组分为：1 瓶冷冻 Expi293F GnTI 细胞、1 L Expi293 表达培养基、1 个足以转染 1 L 培养物的 ExpiFectamine 293 转染试剂盒、100 mL Opti-Plex 络合缓冲液、1 个 PNGase F 聚糖切割试剂盒和 pRABBIT IgG IRES-EmGFP 阳性对照载体。Expi293 GnTI 表达系统的所有组分也可单独购买。

仅供科研使用。不可用于诊断程序。

细胞系Expi293F™

输送类型转染

适用于（应用）蛋白质生产

产品线Expi293™

数量1 kit

运输条件经批准可在室温下或者用湿冰或干冰运输

Unit SizeEach

内容与储存

• Expi293F GnTI 细胞（1 x 10⁷ 个细胞/小瓶），储存于汽相液氮中
• 1 L Expi293 表达培养基，储存于 2–8°C（避光储存）
• ExpiFectamine 293 转染试剂盒，用于 1 L 培养，储存于 2–8°C
• 100 mL Opti-Plex 络合缓冲液，储存于 2–8°C（避光储存）
• PNGase F 聚糖切割试剂盒，储存于 -20°C
• pRABBIT IgG IRES-EmGFP 阳性对照载体，储存于 2–8°C 下或长期存储于 20°C 下

常见问题解答 (FAQ)

我能让Expi293F培养细胞表达蛋白长达7天以上吗？

Expi293F细胞的生长和表达属性决定了在转染7天之后，培养基将临近耗竭，并应该已经达到最高蛋白表达量。继续孵育将导致培养细胞活力的显著下降。

Can I leave my Expi293F culture expressing for more than 7 days?

The growth and expression characteristics of Expi293F cells are such that, by 7 days post-transfection, the culture medium should be close to being spent and maximal protein expression should have already been achieved. Continued incubation will result in a large decrease in cell culture viability.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What method do you recommend for quantifying rabbit IgG expression from pRABBIT IgG IRES-EmGFP Positive Control Vector, Cat. No. A39243?

The formation of intact IgG molecules may be quantified using a sandwich ELISA designed to capture and detect rabbit IgG. Besides the rabbit IgG positive control vector, reagents, and consumables that are included in the kit, you will also need purified rabbit IgG to be used as a standard, F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Cat. No. A10547), Protein A Coated Plates, Clear, 96-Well (Cat. No. 15130), TMB Substrate Kit (Cat. No. 34021), SuperBlock (TBS) Blocking Buffer (Cat. No. 37535), and PBS or TBS buffer for washes. There is an example procedure in our Protein A Coated Plates manual (https://tools.thermofisher.com/content/sfs/manuals/MAN0011310_Thermo Scientific_ProteinA_G_AG_Coat_96Well_UG.pdf). Please note that our R&D scientists determine titer values from crude cell culture supernatants using a Pall Life Sciences FortéBio Octet instrument equipped with a Protein A biosensor.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Do you offer a GFP-expressing mammalian expression vector that I can use as a control to monitor my transfection and expression with the Expi systems?

We offer pRABBIT IgG IRES-EmGFP Positive Control Vector, Cat. No. A39243, which you can use to monitor your transfection and expression.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.