Gibco™

Expi293™ GnTI 表达系统试剂盒

货号: A39250CN
Gibco™

Expi293™ GnTI 表达系统试剂盒

货号: A39250CN
货号
A39250CN
单位规格
1 kit
价格(CNY)
价格 33,937.00
您的价格
库存状况
***
数量
货号单位规格价格(CNY)库存状况数量
A39250CN1 kit
价格 33,937.00
您的价格
***
产品概述
图表
视频
推荐产品
推荐产品
文件
常见问题解答
引用和文献
其他信息
推荐产品
The Expi293 GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The Expi293F GnTI- cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.

Expi293 GnTI- Expression System Kit features include:
• Homogeneous N-glycosylation of expressed proteins
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• Significantly higher protein yields than other HEK293 GnTI- cell line alternatives
• Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

The components included in the Expi293 GnTI- Expression System Kit are: one vial of frozen Expi293F GnTI- cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one PNGase F Glycan Cleavage kit, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 GnTI- Expression System are also available for individual purchase.

规格

输送类型
转染
运输条件
经批准可在室温下或者湿冰或干冰上运输
细胞系
Expi293F™
产品线
Expi293™
适用于(应用)
蛋白质生产

内容与储存

• Expi293F GnTI 细胞(1 x 107 个细胞/小瓶),储存于汽相液氮中
• 1 L Expi293 表达培养基,储存于 2–8°C(避光储存)
• ExpiFectamine 293 转染试剂盒,用于 1 L 培养,储存于 2–8°C
• 100 mL Opti-Plex 络合缓冲液,储存于 2–8°C(避光储存)
• PNGase F 聚糖切割试剂盒,储存于 -20°C
• pRABBIT IgG IRES-EmGFP 阳性对照载体,储存于 2–8°C 下或长期存储于 20°C 下

图表

文件和下载

证书

    常见问题解答 (FAQ)

    引用和文献

    Search citations by name, author, journal title or abstract text