The Expi293 GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The Expi293F GnTI- cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.
Expi293 GnTI- Expression System Kit features include: • Homogeneous N-glycosylation of expressed proteins • Equivalent protein yields to parental Expi293F cells—up to 1 g/L • Significantly higher protein yields than other HEK293 GnTI- cell line alternatives • Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium • High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers
The components included in the Expi293 GnTI- Expression System Kit are: one vial of frozen Expi293F GnTI- cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one PNGase F Glycan Cleavage kit, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 GnTI- Expression System are also available for individual purchase.
规格
输送类型
转染
运输条件
经批准可在室温下或者湿冰或干冰上运输
细胞系
Expi293F™
产品线
Expi293™
适用于(应用)
蛋白质生产
内容与储存
• Expi293F GnTI 细胞(1 x 107 个细胞/小瓶),储存于汽相液氮中 • 1 L Expi293 表达培养基,储存于 2–8°C(避光储存) • ExpiFectamine 293 转染试剂盒,用于 1 L 培养,储存于 2–8°C • 100 mL Opti-Plex 络合缓冲液,储存于 2–8°C(避光储存) • PNGase F 聚糖切割试剂盒,储存于 -20°C • pRABBIT IgG IRES-EmGFP 阳性对照载体,储存于 2–8°C 下或长期存储于 20°C 下