Pierce™ DTT(二硫苏糖醇),No-Weigh™ 规格
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Pierce™ DTT(二硫苏糖醇),No-Weigh™ 规格
Thermo Scientific™

Pierce™ DTT(二硫苏糖醇),No-Weigh™ 规格

Thermo Scientific Pierce DTT(二硫苏糖醇)以 7.7 mg x 48了解更多信息
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货号数量
A3925548 x 7.7 mg
货号 A39255
价格(CNY)
1,667.00
飞享价
Ends: 31-Dec-2025
2,433.00
共减 766.00 (31%)
Each
添加至购物车
数量:
48 x 7.7 mg
价格(CNY)
1,667.00
飞享价
Ends: 31-Dec-2025
2,433.00
共减 766.00 (31%)
Each
添加至购物车
Thermo Scientific Pierce DTT(二硫苏糖醇)以 7.7 mg x 48 管微孔板的形式提供,便于制备 100 μ L 的 0.5M 库存,用于添加到蛋白质样品,实现二硫键的有效还原。

二硫苏糖醇是一种常见的还原剂,适用于多种蛋白研究应用,包括减少聚丙烯酰胺电泳 (SDS-PAGE)。No-Weigh 规格包括一个 48-微量管托盘,其中含有干燥、稳定、单次使用的等份二硫苏糖醇 (DTT)。只需穿刺一个微量管的密封件,并添加 100 μL 的水或缓冲液即可。您只需几秒即可获得可用于多种实验室方法的 500 mM DTT 溶液。根据需要稀释或添加适当体积的 500 mM 储备液,以达到特定应用所需的还原剂量。

(No-Weigh 规格的)特点:

节省时间—只需加入 100 μL 水,即可在数秒内制备 500 mM DTT 溶液
消除浪费—一次制备 100 μL,正是针对大多数应用的正确量
始终新鲜—确保每次使用都具有完全还原强度;无需储存储备液

使用便利的 No-Weigh 规格,可在不到 30 秒的时间内制备 500 mM DTT 溶液。独特的包装可确保还原剂具有完全强度,并能够在电泳或其他应用前保护蛋白免受氧化损伤或还原任何二硫化物。

相关产品
防粘结材料™ TCEP 溶液,中性 pH 值
仅供科研使用。不可用于诊断程序。
规格
产品线Pierce™
数量48 x 7.7 mg
Unit SizeEach
内容与储存
Upon receipt, store product at 4°C.

常见问题解答 (FAQ)

What is the shelf life of Pierce DTT (Dithiothreitol), No-Weigh Format (Cat. No. A39255)?

Pierce DTT (Dithiothreitol), No-Weigh Format is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale for more details.

Can you please recommend a method for reduction, alkylation, and digestion of an antibody?

Prepare 1 mg/mL antibody in 7.0 M Guanidine HCl, 100 mM Tris, pH 8.3. For reduction, add DTT (Cat. Nos. A39255, 20290) solution at 10 mM final concentration and incubate for 30 mins at room temperature. For alkylation, add sodium iodoacetate at 20 mM final concentration and incubate in the dark for 20 mins at room temperature. Perform buffer exchange followed by digestion using Pierce MS grade Trypsin, Cat. Nos. 90058, 90057 (1:10 enzyme to substrate ratio).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.