EZ-Link™ Sulfo-NHS-生物素
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EZ-Link™ Sulfo-NHS-生物素
引用和文献 (10)
Thermo Scientific™

EZ-Link™ Sulfo-NHS-生物素

Thermo Scientific EZ-Link Sulfo-NHS-生物素是一种短链、水溶性生物素化试剂，用于标记具有伯胺的抗体、蛋白和其他分子。EZ-Link Sulfo-NHS-生物素的特点：•蛋白标记—生物素化抗体便于使用链霉素亲和素树脂或探针进行固定、纯化或检测•细胞表面标记—仅对全细胞的表面蛋白进行生物素化，因为带负电荷的试剂不会渗透细胞膜• 胺反应性—与伯胺了解更多信息
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货号数量
A3925610 x 1 mg
2121750 mg
货号 A39256
价格（CNY)
1,387.00
飞享价
Ends: 31-Dec-2025
2,023.00
共减 636.00 (31%)
Each
添加至购物车
数量:
10 x 1 mg
价格（CNY)
1,387.00
飞享价
Ends: 31-Dec-2025
2,023.00
共减 636.00 (31%)
Each
添加至购物车
Thermo Scientific EZ-Link Sulfo-NHS-生物素是一种短链、水溶性生物素化试剂，用于标记具有伯胺的抗体、蛋白和其他分子。

EZ-Link Sulfo-NHS-生物素的特点：

•蛋白标记—生物素化抗体便于使用链霉素亲和素树脂或探针进行固定、纯化或检测
•细胞表面标记—仅对全细胞的表面蛋白进行生物素化，因为带负电荷的试剂不会渗透细胞膜
• 胺反应性—与伯胺 (-NH2)（如赖氨酸侧链或多肽的氨基末端）发生反应
• 可溶性—与普通 NHS 酯化合物相比带电荷的 sulfo-NHS 基团可提高试剂的水溶性
• 不可逆—形成永久酰胺键；间隔臂不可切割
• 非常短—间隔臂（添加至靶标的总长度）为 13.5 埃，该臂仅含有天然生物素戊酸基团。

Sulfo-NHS-生物素是三种非常相似的 EZ-Link 试剂中最短的一种，该试剂具有水溶性，不可切割，且在溶液中可实现抗体、蛋白和任何其他含伯胺大分子的简单高效生物素化。对于这些独特的水溶性、膜不渗透性试剂，细胞表面蛋白的特异性标记是另一种常见应用。只有间隔臂长度不同，三种 Sulfo-NHS 酯试剂可优化标记和检测实验，实验中生物素结合的空间位阻是重要因素。Sulfo-NHS-生物素提供为多种包装规格和完整的蛋白标记试剂盒。

我们生产生物素试剂，以确保尽可能使预期研究应用的总体产品完整性、一致性和性能达到较高水平。

生物素 N-羟基磺基琥珀酰亚胺 (NHS) 酯是广泛采用的生物素化试剂类型。NHS-活化生物素在碱性缓冲液中与伯胺基 (-NH2) 高效反应，形成稳定的酰胺键。蛋白（如抗体）通常有几种可作为标记靶标的伯胺，包括赖氨酸 (K) 残基的侧链和每个多肽的 N-末端。

生物素各种 NHS 酯试剂在长度、溶解度、细胞通透性和可切割性方面有所不同。非磺化 NHS-生物素具有细胞渗透性，但必须在有机溶剂（如 DMSO 或 DMF）中溶解。Sulfo-NHS 生物素（和带有聚乙二醇化间隔的生物素）可以直接溶解在水中，但无膜渗透性。含二硫键的各种类型可使用还原剂进行切割，从而使生物素基团与标记蛋白分离。

相关产品
EZ-Link™Sulfo-NHS-生物素化试剂盒
EZ-Link™微型 Sulfo-NHS-生物素化试剂盒
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性细胞不可透过性
套装内容10 x 1 mg
标记方法化学标记
产品线EZ-Link
产品类型Sulfo-NHS-生物素
数量10 x 1 mg
反应一部分Sulfo-NHS 酯
运输条件经检验可环境温度或湿冰运输
化学反应性胺
标签或染料生物素
溶解度DMF（二甲基甲酰胺）, DMSO（二甲亚砜）, 水
间隔子短
Unit SizeEach
内容与储存
在 -20°C 下干燥储存。

常见问题解答 (FAQ)

Can you provide the shelf-life for EZ-Link Sulfo-NHS-Biotin?

EZ-Link Sulfo-NHS-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (-20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

引用和文献 (10)

引用和文献
Abstract
Investigation of pre-existing reactivity to biotherapeutics can uncover potential immunogenic epitopes and predict immunogenicity risk.
Authors:Bivi N, Moore T, Rodgers G, Denning H, Shockley T, Swearingen CA, Gelfanova V, Calderon B, Peterson DA, Hodsdon ME, Siegel RW, Higgs RE, Konrad RJ
Journal:MAbs
PubMed ID:31099718
'Despite recent advances in the development of tools to predict immunogenicity risk of biotherapeutic molecules, the ability of a protein to elicit the formation of anti-drug antibodies (ADA) remains one of the most common causes for termination of clinical development programs. In this study, we use ADA assays to detect ... More
Interplay of the Norrin and Wnt7a/Wnt7b signaling systems in blood-brain barrier and blood-retina barrier development and maintenance.
Authors:Wang Y, Cho C, Williams J, Smallwood PM, Zhang C, Junge HJ, Nathans J
Journal:Proc Natl Acad Sci U S A
PubMed ID:30478038
'ß-Catenin signaling controls the development and maintenance of the blood-brain barrier (BBB) and the blood-retina barrier (BRB), but the division of labor and degree of redundancy between the two principal ligand-receptor systems-the Norrin and Wnt7a/Wnt7b systems-are incompletely defined. Here, we present a loss-of-function genetic analysis of postnatal BBB and BRB ... More
Beneficial Role of Rosuvastatin in Blood-Brain Barrier Damage Following Experimental Ischemic Stroke.
Authors:Lu D, Mai HC, Liang YB, Xu BD, Xu AD, Zhang YS
Journal:Front Pharmacol
PubMed ID:30186167
'Hemorrhage transformation is the most challenging preventable complication in thrombolytic therapy and is related to recombinant tissue plasminogen activator (rt-PA)-induced blood-brain barrier (BBB) damage. Intraperitoneal injections of normal or high doses of rosuvastatin were administered to Balb/c mice 20 min prior to middle cerebral artery occlusion (MCAO) surgery for 3 ... More
Severe, but not mild heat-shock treatment induces immunogenic cell death in cancer cells.
Authors:Adkins I, Sadilkova L, Hradilova N, Tomala J, Kovar M, Spisek R
Journal:Oncoimmunology
PubMed ID:28638734
The mechanisms of immunogenicity underlying mild heat-shock (mHS) treatment < 42°C of tumor cells are largely attributed to the action of heat-shock proteins; however, little is known about the immunogenicity of tumor cells undergoing severe cytotoxic heat-shock treatment (sHS > 43°C). Here, we found that sHS, but not mHS (42°C), ... More
Mechanotransduction by PCDH15 Relies on a Novel cis-Dimeric Architecture.
Authors:Dionne G, Qiu X, Rapp M, Liang X, Zhao B, Peng G, Katsamba PS, Ahlsen G, Rubinstein R, Potter CS, Carragher B, Honig B, Müller U, Shapiro L
Journal:Neuron
PubMed ID:30057206
The tip link, a filament formed by protocadherin 15 (PCDH15) and cadherin 23, conveys mechanical force from sound waves and head movement to open hair-cell mechanotransduction channels. Tip-link cadherins are thought to have acquired structural features critical for their role in mechanotransduction. Here, we biophysically and structurally characterize the unusual cis-homodimeric ... More
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