Pierce™ 烷基化试剂
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Pierce™ 烷基化试剂
Pierce™ 烷基化试剂
Pierce™ 烷基化试剂
引用和文献 (2)
Thermo Scientific™

Pierce™ 烷基化试剂

以一次性预称重规格提供的烷基化试剂可简化蛋白制备工作流程。
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货号数量包括
A3927130 x 9.3 mg碘乙酰胺，No-Weigh™ 规格
A3927010 x 2 mg氯乙酰胺，No-Weigh™ 规格
货号 A39271
价格（CNY)
1,752.00
飞享价
Ends: 31-Dec-2025
2,529.00
共减 777.00 (31%)
Each
添加至购物车
数量:
30 x 9.3 mg
包括:
碘乙酰胺，No-Weigh™ 规格
价格（CNY)
1,752.00
飞享价
Ends: 31-Dec-2025
2,529.00
共减 777.00 (31%)
Each
添加至购物车
Thermo Scientific Pierce 碘乙酰胺或氯乙酰胺以 No-Weigh 规格提供，其中少量试剂经过预先称重和包装以供一次性使用。这种一次性规格可防止随时间推移因重复打开和关闭单个样品瓶而造成试剂污染和反应性损失。这种规格可让用户每次都使用一瓶新鲜试剂，而无需麻烦地称取少量试剂。
碘乙酰胺和氯乙酰胺烷基化试剂是巯基反应性烷基化试剂，用于封闭还原的半胱氨酸残基，以进行蛋白表征和肽图谱分析。半胱氨酸还原后使用碘乙酰胺和氯乙酰胺进行烷基化会导致共价添加脲甲基基团 (57.07 Da)，从而防止二硫键形成。烷基化后添加的还原剂将与多余的碘乙酰胺发生反应。
For Research Use Only. Not for use in diagnostic procedures.
规格
包括碘乙酰胺，No-Weigh™ 规格
产品线Pierce
数量30 x 9.3 mg
产品类型烷基化试剂
Unit SizeEach

常见问题解答 (FAQ)

What is the purity level for Pierce Iodoacetamide (Cat. No. A39271)?

The purity of Pierce Iodoacetamide, measured by NMR, is 99%.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

引用和文献 (2)

引用和文献
Abstract
Clean and Complete Protein Digestion with an Autolysis Resistant Trypsin for Peptide Mapping.
Authors:Muriithi B,Ippoliti S,Finny A,Addepalli B,Lauber M
Journal:Journal of proteome research
PubMed ID:39392678
Peptide mapping requires cleavage of proteins in a predictable fashion so that target protein-specific peptides can be reliably identified and quantified. Trypsin, a commonly used protease in this process, can also undergo self-cleavage or autolysis, thereby reducing the effectivity and even cleavage specificity at lysine and arginine residues. Here, we ... More
Proximity-CLIP and Expedited Non-Radioactive Library Preparation of Small RNA Footprints for Next-Generation Sequencing.
Authors:Anastasakis D,Benhalevy D,Hafner M
Journal:Current protocols in molecular biology
PubMed ID:32438484
During the course of their life cycle, most RNAs move between several cellular environments where they associate with different RNA binding proteins (RBPs). Reciprocally, a significant portion of RBPs reside in more than a single cellular compartment, where they can interact with discrete RNAs and even exert distinct biological roles. ... More