Ion Torrent™ 双条形码试剂盒 1-96
Ion Torrent™ 双条形码试剂盒 1-96
Ion Torrent™

Ion Torrent™ 双条形码试剂盒 1-96

Ion Torrent 双条形码试剂盒件 1-96 提供 96 种独特的双匹配条形码接头,专门设计用于实现扩增子文库和 Ion 半导体测序仪的理想性能。当与了解更多信息
Have Questions?
货号数量
A393601 个试剂盒
货号 A39360
价格(CNY)
-
数量:
1 个试剂盒
Ion Torrent 双条形码试剂盒件 1-96 提供 96 种独特的双匹配条形码接头,专门设计用于实现扩增子文库和 Ion 半导体测序仪的理想性能。当与 Ion AmpliSeq 和 Oncomine 试剂盒结合使用时,该试剂盒用户能够将 96 份扩增子文库合并以进行多重测序分析,从而提高实验室效率并降低每份样品的测序成本。条形码接头以 96 孔板形式提供。

Ion Torrent 双条形码试剂盒 1-96 的特点包括:
•使用稳健的分子条形码,可在单个测序芯片上对大量扩增子文库样品进行倍增以鉴别极罕见克隆,具有高准确度
•经序列和流式优化,可在池中平均显示所有条形码,同时进行更经济的多重测序运行
•通过设计内置的稳健误差校正功能增加了样品鉴别的可信度

罕见克隆的较高通量和变异检测
通过双条形码文库进行多重检测可分析多达 96 份样品/运行、降低测序成本和处理要求,实现成本节约的运行。对各条形码单独检测以最大限度降低呈现偏倚并在检测低频克隆检测中提供卓越灵敏度,具有高准确度。

进行序列和流式优化以提高性能和效率
条形码接头包含经过优化的序列,以在池中平均显示所有条形码,需要最低数量的流量即可进行查询,因此获得更经济的多重测序运行。

通过设计内置的稳健误差校正
内置误差校正可以增加样品分配的准确度。
仅供科研使用。不可用于诊断程序。
规格
适用于(应用)测序
适用于(设备)Ion GeneStudio™ S5 Plus 系统、Ion GeneStudio™ S5 引物系统、Ion GeneStudio™ S5 系统
产品线Ion Torrent™
产品类型双条形码试剂盒
数量1 个试剂盒
样品类型DNA
运输条件室温
工作流程步骤文库条形码
产品规格试剂盒
测序类型基因组和 DNA 测序
Unit SizeEach
内容与储存
96 孔板,每孔单次使用 (6 μL),储存在冰箱中(-5 至 -30°C)

常见问题解答 (FAQ)

How many Ion AmpliSeq Mouse TCR Beta SR Assay, RNA libraries can I load on each type of chip?

For the Ion 530 Chip, 8 libraries can be loaded. For the Ion 540 Chip, the number of libraries that can be loaded ranges from 8 to 32, depending on the size of the libraries. For the Ion 550 Chip, the number of libraries that can be loaded ranges from 12 to 48.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What types of chips can I use with the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA?

The Ion AmpliSeq Mouse TCR Beta SR Assay, RNA is compatible with the Ion 530 Chip, Ion 540 Chip, and Ion 550 Chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA?

The Ion AmpliSeq Mouse TCR Beta SR Assay, RNA (Cat. No. A45489) consists of the Ion AmpliSeq Mouse TCR Beta-SR Panel, the Ion AmpliSeq Library Kit Plus (Cat. No. 4488990), and the Ion Torrent NGS Reverse Transcription Kit (Cat. No. A45003). Sufficient reagents are provided for the rapid preparation of 24 sample libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How much of each sample is needed for the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA?

The sample RNA input can range from 25 ng to 1 µg.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What types of samples is the Ion AmpliSeq Mouse TCR Beta SR Assay, RNA compatible with?

The Ion AmpliSeq Mouse TCR Beta SR Assay, RNA is compatible with a vast array of research sample types, including FFPE tissue, fresh-frozen (FF) tissue, sorted T cells, whole blood, periperal blood leukocytes (PBLs), and periperal blood mononuclear cells (PBMCs).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.