EquiPhi29™ DNA 聚合酶
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EquiPhi29™ DNA 聚合酶
Thermo Scientific™

EquiPhi29™ DNA 聚合酶

Thermo Scientific EquiPhi29 DNA 聚合酶是一种专有的通过体外蛋白进化形成的 phi29 DNA 聚合酶突变体。通过 phi29了解更多信息
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货号数量ProductName
A39390250 U,10 U/μL
A393911000 U,10 U/μL
A393925000 U,10 U/μL
货号 A39390
价格(CNY)
631.00
飞享价
Ends: 31-Dec-2025
902.00
共减 271.00 (30%)
Each
添加至购物车
数量:
250 U,10 U/μL
请求批量或定制报价
价格(CNY)
631.00
飞享价
Ends: 31-Dec-2025
902.00
共减 271.00 (30%)
Each
添加至购物车
Thermo Scientific EquiPhi29 DNA 聚合酶是一种专有的通过体外蛋白进化形成的 phi29 DNA 聚合酶突变体。通过 phi29 DNA 聚合酶,这种酶的蛋白质的热稳定性、反应速度、产物产量和扩增偏移均得到了显著改善,同时保留了野生型酶的所有优势,包括高持续合成能力(大于 70 kb)、很强的链置换活性和优先作用在单链 DNA 或 RNA 上 3'→5' 核酸外切酶(校正读码)活性。因此,推荐使用抗水解的随机引物。

产品优势包括:
•市场上最低的扩增偏差
• 较高得率的扩增 DNA,即使用微量模板也是如此
• 在更短的时间内实现高度准确的 DNA 合成

应用:
•使用多种样品类型的无偏全基因组扩增 (WGA):
   –来自单细胞的 DNA
   –未培养的微生物细胞和病毒颗粒
   –病原微生物或宏基因组
   –用于 SNP 和 STR 检测的 DNA
• 滚环扩增 (RCA)
• 蛋白引物 DNA 扩增
• 致命性 DNA 的无细胞克隆
• 采用挂锁探针的原位基因分型
• RNA 引物 DNA 扩增

注:向含 EquiPhi29 DNA 聚合酶的反应混合物加入焦磷酸酶可能会进一步增强 DNA 的合成。

仅供科研使用。不可用于诊断程序。
规格
最大浓度10 U/μL
最佳反应温度42°C
反应时间2 hours
运输条件干冰
适用于(应用)MDA-WGA, RCA
聚合酶EquiPhi29 DNA 聚合酶
产品类型Stand-alone enzyme
数量250 U,10 U/μL
Unit SizeEach
内容与储存
• 25 μL EquiPhi29 DNA 聚合酶 (10 U/μL)
• 0.25 mL EquiPhi29 DNA 聚合酶反应缓冲液 (10X)
• 0.25 mL DTT (100 mM)

在 -5 至 -30°C 下储存。

常见问题解答 (FAQ)

What is the error rate of EquiPhi29 DNA Polymerase?

The error rate of EquiPhi29 DNA Polymerase is 6 x 10-6.
The error rate of EquiPhi29 DNA Polymerase was measured according to the method described in literature:
Mielinis, P., Sukackaitė, R., Serapinaitė, A., Samoilovas, F., Alzbutas, G., Matjošaitis, K., & Lubys, A. (2021). MUA-based molecular indexing for rare mutation detection by Next-Generation sequencing. Journal of Molecular Biology, 433(19), 167209. https://doi.org/10.1016/j.jmb.2021.167209

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I use EquiPhi29 DNA Polymerase to incorporate 5-methyl-dCTP?

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary mutant phi29 DNA Polymerase developed through in vitro protein evolution. EquiPhi29 DNA Polymerase as well as phi29 DNA Polymerase should be able to incorporate 5-methyl-dCTP nucleotides and other modified nucleotides.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

When using EquiPhi29 DNA Polymerase, do I need to purify amplified DNA products before downstream applications?

Cleaning of the amplified product is not required prior to several downstream methods (e.g., debranching, digestion with restriction endonucleases, Sanger sequencing); the dilution of amplified product is sufficient. If the clean-up procedure is needed, we recommend using an affinity-based spin-column or magnetic bead-based purification method.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

What is the minimal recommended time for amplification with EquiPhi29 DNA Polymerase?

The optimal reaction time for DNA amplification with EquiPhi29 DNA Polymerase is 2 hours. For samples with ≥1 pg of DNA input, DNA amplification time can be shortened to 1 hour if maximizing product yield is not essential.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can liquid media culture or colonies be used as a starting material for amplification with EquiPhi29 DNA Polymerase?

Yes. EquiPhi29 DNA Polymerase can work with different types of sample input material such as purified DNA, liquid media culture, agar plate colonies, etc.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.