Ponceau S Staining Solution - FAQs

查看更多产品信息 Ponceau S Staining Solution - FAQs (A40000278, A40000278X3, A40000279)

2 个常见问题解答

What is the best way to image Ponceau staining with an iBright Imaging System?

The recommended imaging method depends on the iBright imager model being used. For iBright FL1500 and CL1500 Imaging Systems, we recommend imaging Ponceau-stained samples with the Ponceau application that can be found in Universal mode. For the iBright CL750 Imaging System, we recommend imaging Ponceau-stained samples in Protein gel mode.

We always recommend imaging with the orange iBright System Contrast Tray (Cat. No. A56597), and if needed, adjust the focus or zoom to get bands as sharp as possible. Better quality pictures are obtained when the membrane is wet.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the No-Stain Protein Labeling Reagent with an orange or red dye (i.e., will the signal appear in channel 2, 3, 4 or 5, not just channel 1 in the iBright imager)?

Channels 3, 4 and 5 on the iBright imager can be used for multiplexing without interference while using the No-Stain Protein Labeling Reagent. Channels 1 and 2 on the iBright imager will show interference. Fluorophores for multiplexing that are compatible with the No-Stain Protein Labeling Reagent are listed here, under the “Secondary Abs” tab (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/total-protein-normalization.html).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.