Geltrex™ Flex LDEV-Free Organoid-Qualified Reduced Growth Factor Basement Membrane Matrix

Gibco Geltrex Flex LDEV-Free Organoid-Qualified Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane that is purified from Engelbreth-Holm-Swarm (EHS) tumor cells. The major components of Geltrex Flex matrix include laminin, collagen IV, entactin, and heparan sulfate proteoglycan.

Recommended for culturing tumoroids and other organoids, organoid-qualified Geltrex Flex matrix was used to grow human lung cancer tumoroids and human colorectal cancer tumoroids in suspension and embedded culture using the OncoPro Tumoroid Culture Medium Kit. For a detailed description of OncoPro tumoroid culture, see the OncoPro Tumoroid Culture Medium Kit User Guide (Pub. No. MAN0029372). To help ensure support of organoid culture, every lot of organoid-qualified Geltrex Flex matrix is quality control–tested for stable dome formation and growth of multiple mouse organoids.

Features of Geltrex Flex LDEV-Free Organoid-Qualified Reduced Growth Factor Basement Membrane Matrix

• Lots tested to help ensure growth of mouse intestinal, liver, ductal, and airway organoids and the expansion of mouse small intestinal organoids for more than five passages with typical organoid budding morphology
• Each lot tested for the absence of lactose dehydrogenase elevating virus (LDEV), mycoplasma, and a broad panel of viruses and microorganisms
• Lots tested for endotoxin, maintenance of gel stability, and HUVEC tube formation
• Growth factor–reduced to improve lot-to-lot consistency
• Formulated without phenol red to minimize potential for estrogen-like effects
• Provided in recyclable glass vials
• 1-mL, 5-mL, 10-mL sizes for maximum flexibility. Use only what you need. Reduce need for extensive aliquoting and unwanted freeze-thaws.

For the culture of primary cells, such as epithelial cells, endothelial cells, smooth muscle cells, and angiogenesis assays, neurite outgrowth assays, or bile canaliculi formation assays with polarized hepatocytes, standard Geltrex Flex matrix can be used.

For culturing hESCs or iPSCs, it is recommended to use Geltrex Flex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix. Each lot of hESC-qualified Geltrex Flex matrix is tested with iPSCs and H9 hESCs for at least five passages to help ensure reliability and consistency in hESC/iPSC studies.