VetMAX M. paratuberculosis 3.0 Kit

The Applied Biosystems VetMAX M. paratuberculosis 3.0 Kit utilizes real-time PCR for the sensitive and specific detection of Mycobacterium avium ssp. paratuberculosis (MAP) nucleic acid in animal samples, allowing for reliable and rapid detection of infected animals. The kit can be used to test for MAP in many species (cattle, sheep, goats, and camels) and a wide variety of sample types (feces, milk, environmental samples).

Key features and benefits

• Complete solution—this kit is part of a complete workflow. For all products in one purchase (consumables, sample prep, assay) see our VetMAX M. paratuberculosis 3.0 Toolbox (Cat. No. A40004116).
• Flexibility—validated for multiple species and sample types; pooling for fecal samples with solution including lysis/stability/no foam capacity; high- and low-throughput protocols
• Streamlined disruption protocol—the assay is validated alongside our MagMAX CORE disruption modules, which now have fewer sample preparation steps
• Faster—compatible with express extraction (~21 min) and fast amplification (<1 h) programs; includes a ready-to-use master mix
• High-throughput disruption option—the MagMAX CORE Disruption Plates Module contains deep-well plates prefilled with zirconia beads, helping enable quick processing of >96 samples with no need for individual screw top tubes
• Sustainability—less steps = less tips; high-throughput packaging of 500 reactions limits plastics, boxes, and size of shipments/stockage

The VetMAX M. paratuberculosis 3.0 Kit allows optimal sensitivity across all sample types in a short 54-minute amplification protocol. The test has been optimized to ensure consistent performance while also benefiting from a fast turnaround time for results.

The VetMAX M. paratuberculosis 3.0 assay kit, in combination with our MagMAX CORE sample disruption products (Cat. Nos. A40004818 and A40004819) and nucleic acid purification kit, enables a workflow that has been fine tuned to provide labs with a fast, user-friendly, complete process for high throughout processing with enhanced performance. Internal and external positive controls are provided with the kit to help provide confidence and standardization of results.

MAP infection, also known as Johne's Disease, primarily targets the mesenteric ganglia and small intestine, leading to a chronic intestinal infection. Clinical symptoms include diarrhea, weight loss, and edema. These symptoms become noticeable only in the advanced stages of the disease. The main diagnostic tools include PCR and ELISA tests (see Cat. Nos. A65415, A65415 for ELISA options).