EasyPep™ MS 样品制备试剂盒
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EasyPep™ MS 样品制备试剂盒
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EasyPep™ MS 样品制备试剂盒

使用经优化的 EasyPep MS 样品制备试剂盒在不到四小时内生成用于 MS 分析的优质蛋白组学样品。
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货号描述
A40006微型 MS 样品制备试剂盒
A57864Micro MS Sample Prep Kit
A45733MS 样品制备试剂盒
A45734大型样品制备试剂盒
A45735裂解缓冲液
A57865Peptide Clean-up Plate
货号 A40006
价格（CNY)
6,114.00
飞享价
Ends: 31-Dec-2025
7,916.00
共减 1,802.00 (23%)
Each
添加至购物车
描述:
微型 MS 样品制备试剂盒
价格（CNY)
6,114.00
飞享价
Ends: 31-Dec-2025
7,916.00
共减 1,802.00 (23%)
Each
添加至购物车

EasyPep Mini MS 样品制备试剂盒可对培养的哺乳动物细胞和组织进行高效、可重现的处理以便进行蛋白组学 MS 分析。该试剂盒包含预配制的缓冲液、MS 级酶混合物、肽纯化柱和优化的方案，可在 3 小时内生成质谱兼容肽样品。试剂盒经过优化，可处理 10–100 μg 的蛋白样品，产生大量的 MS 即用型肽。

• 全面—包含用于酶切裂解的预配制试剂、肽纯化柱和用于多达 20 份样品的优化方案
• 经过优化—简化方案和试剂，尽可能减少处理样品所需的步骤和时间
• 灵活—试剂和方案已通过 10-100 µg 样品量的细胞、血浆和组织样品进行验证
• 节省时间—样品处理时间从一天以上缩短至不到三小时
• 兼容—最终制剂可用于直接 MS 分析和其他下游应用，包括 TMT 标记

用于 MS 分析的肽样品制备较复杂，步骤繁多，方案多变，导致肽的质量和数量都存在差异。本试剂盒设计用于提高重现性，同时节省手动操作和处理时间。通过添加通用核酸酶以降低来自核酸的粘度（替代超声处理）、用于半胱氨酸修饰的快速“一锅法”还原/烷基化溶液（脲甲基化，+ 57.02）和用于更完全酶切的胰蛋白酶/Lys-C 蛋白酶混合物，减少了步骤数量和处理时间。此外，该试剂盒还包括肽纯化柱和缓冲液，用于制备不含去污剂的肽样品进行 MS 分析。优化的试剂和方案可以产生兼容 TMT 标记和其他下游应用，或即时用于 MS 分析的高质量肽。

For Research Use Only. Not for use in diagnostic procedures.
规格
描述微型 MS 样品制备试剂盒
最终产品类型肽
适用于（设备）质谱仪
数量20 次反应
运输条件湿冰
工作流程步骤细胞裂解
检测方法质谱分析
产品线EasyPep™
产品类型样品制备试剂盒
原始材料蛋白样品
Unit SizeEach

常见问题解答 (FAQ)

Are the EasyPep MS Sample Prep Kits compatible with vacuum pumps/dry-down devices?

As per protocol, peptide samples need to be dried down prior to LC-MS analysis. This step can be performed using vacuum pumps/dry-down devices without any harm caused to the device by the components present in the elution buffer within the EasyPep MS Sample Prep Kits.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

What is the composition of the Lysis Solution in the EasyPep MS Sample Prep Kits (Cat. Nos. A4006, A45733, A5734, A45735)?

The composition of the Lysis Solution in the EasyPep MS Sample Prep Kits (Cat. Nos. A4006, A45733, A5734, A45735) is proprietary. The kit includes pre-formulated buffers, MS-grade enzyme mix, and peptide columns to prepare high-quality detergent-free peptide samples for direct LC-MS analysis or further sample processing such as isobaric tag (e.g., TMT or TMT pro reagent) labeling, phosphopeptide enrichment, or high-pH reversed-phase fractionation.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can I prepare my samples in Laemmli sample buffer for use with EasyPep MS Sample Prep Kits (Cat. Nos. A40006, A45733, A45734)?

We have not validated our EasyPep MS Sample Prep Kits (Cat. Nos. A40006, A45733, A45734) for use with Laemmli sample buffer. The lysis buffer provided with the kit has been optimized to be compatible with digestion and clean-up steps. We do not recommend using other buffers as they may contain detergents or other components that may interfere with reduction, alkylation, digestion, or peptide clean-up. The high concentration of SDS in Laemmli sample buffer could potentially disrupt the activity of the universal nuclease.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

After cell lysis, my mass spectrometry sample is very viscous and difficult to pipette. How do I reduce the sample viscosity?

High sample viscosity after lysis is due to release of DNA from the nucleus. Sonication or addition of a nuclease such as the Pierce Universal Nuclease (Cat. No. 88700, 88701, or 88702) can be used to degrade DNA and reduce sample viscosity.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I process yeast samples with EasyPep Mini MS Sample prep Kit?

Add 0.5 mm diameter glass beads to the sample pelleted in Easy Pep Lysis Solution and proceed as recommended in the Easy Pep sample prep protocol. Vortex vigorously for few minutes, spin at 16,000 x g for 5 mins, and extract the supernatant for protein assay and further downstream sample preparation.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all EasyPep™ MS 样品制备试剂盒 FAQs