BenchStable™ DMEM/F12
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BenchStable™ DMEM/F12
Gibco™

BenchStable™ DMEM/F12

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Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) 是一种广泛使用的基础培养基,可用于支持很多种类的哺乳动物细胞生长。已在了解更多信息
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货号数量
A419200210 x 500 mL
A4192001500 mL
货号 A4192002
价格(CNY)
5,661.80
飞享价
Ends: 31-Dec-2025
7,169.00
共减 1,507.20 (21%)
Each
添加至购物车
数量:
10 x 500 mL
价格(CNY)
5,661.80
飞享价
Ends: 31-Dec-2025
7,169.00
共减 1,507.20 (21%)
Each
添加至购物车
Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) 是一种广泛使用的基础培养基,可用于支持很多种类的哺乳动物细胞生长。已在 DMEM/F-12 中成功培养的细胞包括 MDCK、胶质细胞、成纤维细胞、人内皮细胞及小鼠成纤维细胞。我们针对广泛的细胞培养应用,提供了多种组分的 DMEM/F-12 改良培养基。使用培养基选择工具查找适合的配方。

BenchStable DMEM/F-12 经过专门设计,可以在室温下储存,对细胞增殖和最大细胞密度的支持效果可媲美添加 ≥10% 胎牛血清 (FBS) 的常规 DMEM/F12。

这种 DMEM/F-12 的改良方式如下:
•针对室温稳定性进行了优化
• 包含 GlutaMAX、酚红
• 不含 HEPEPS

BenchStable DMEM/F-12 的使用
DMEM/F-12 是一种 1:1 DMEM 和 Ham's F-12 混合物。该配方将 DMEM 的高浓度葡萄糖、氨基酸及维生素与 F-12 的广泛组分结合在一起。DMEM/F-12 使用碳酸氢钠缓冲体系,因此需要 5–10% CO2 的环境来维持生理 pH 值。含有 GlutaMAX 添加剂的 DMEM/F-12 具有易于使用的包装形式,可显著减少有毒氨的积累,并改善细胞活力和生长情况。BenchStable DMEM/F-12 不含蛋白质、脂质或生长因子,因此需要补充 ≥10% 胎牛血清 (FBS)

cGMP 生产和质量体系
Gibco BenchStable 培养基在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在 FDA 登记的医疗器械生产商,且通过 ISO 13485 标准认证。

有关 Gibco BenchStable 培养基的更多信息,请访问 thermofisher.cn/benchstable

仅供科研使用。不可用于诊断程序。
规格
细胞系MDCK、神经胶质细胞、成纤维细胞、人内皮细胞和大鼠成纤维细胞
最大浓度1 X
环保功能节能、使用的资源极少、可持续包装
生产质量cGMP-compliant under the ISO 9001: 2015 standard
产品线BenchStable™
产品类型DMEM(Dulbecco 改良 Eagle 培养基)/F-12
数量10 x 500 mL
有效期自生产之日起 12 个月
运输条件室温
分类非动物源性
形式液体
血清水平标准血清
无菌无菌过滤
加有添加剂高糖, GlutaMAX, 酚红, 丙酮酸钠
不加添加剂不含 HEPES
Unit SizeEach
内容与储存
在室温下储存。避光。

常见问题解答 (FAQ)

Is it okay if I supplement BenchStable media with more than 10% FBS?

We recommended supplementing BenchStable media with at least 10% FBS for best results. If your culture method calls for greater than 10% FBS supplementation using our other existing media, we recommend that you use the same concentration with BenchStable media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it okay if I supplement BenchStable media with 5% FBS?

We recommend supplementing BenchStable media with at least 10% FBS for best results. We have run tests on limited cell types using 5% FBS concentration and have not observed compromised performance. Please contact Tech Support at techsupport@thermofisher.com to ensure that your specific application allows for 5% serum supplementation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which cell lines have been tested in BenchStable media?

We have tested our BenchStable media for growth and morphology on the following cell lines: A549, CHO-K1, HEK293, HeLa, HL-60, Jurkat, MCF7, MDA-MB-231, Neuro2A, SH-SY5Y, and THP-1.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer BenchStable media without phenol red?

At this time, we do not offer BenchStable media without phenol red.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the shelf-life of BenchStable media improve if stored at lower temperature (2-8 degrees C)?

The shelf life of BenchStable media is unchanged when stored cold (2-4 degrees C) or ambient (15-30 degrees C).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.