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View additional product information for BenchStable™ RPMI 1640 - FAQs (A4192301, A4192302)
14 product FAQs found
We recommended supplementing BenchStable media with at least 10% FBS for best results. If your culture method calls for greater than 10% FBS supplementation using our other existing media, we recommend that you use the same concentration with BenchStable media.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We recommend supplementing BenchStable media with at least 10% FBS for best results. We have run tests on limited cell types using 5% FBS concentration and have not observed compromised performance. Please contact Tech Support at techsupport@thermofisher.com to ensure that your specific application allows for 5% serum supplementation.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We have tested our BenchStable media for growth and morphology on the following cell lines: A549, CHO-K1, HEK293, HeLa, HL-60, Jurkat, MCF7, MDA-MB-231, Neuro2A, SH-SY5Y, and THP-1.
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At this time, we do not offer BenchStable media without phenol red.
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The shelf life of BenchStable media is unchanged when stored cold (2-4 degrees C) or ambient (15-30 degrees C).
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We recommend storing BenchStable media under ambient conditions (15-30 degrees C), protected from light. BenchStable media can alternatively be stored at 2-8 degrees C.
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Yes, the box provided with our BenchStable media has properties that offer some resistance to moisture generated in a refrigerator environment.
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Yes, BenchStable media should be stable for use if received partially or completely frozen.
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Our in-house data, using refrigerated BenchStable media exposed to fluorescent light (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/classical-media/benchstable-cell-culture-media.html#results), illustrates the necessity of having light protection. While we have not tested other light spectrums; we recommend storing BenchStable media protected from any light exposure.
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We have observed rapid degradation of several components within days from exposure of BenchStable media to common fluorescent light. We have also observed decreased cell growth using light-exposed media.
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BenchStable media can be used for up to 2-4 weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
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To reduce the chances of contamination and the impact of pH drift, we recommend using BenchStable media within 2-4 weeks after the bottle has been opened.
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Complete BenchStable media (for example, media supplemented with 10% FBS) should be stored at 4 degrees C.
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BenchStable media have a 12 month shelf life, consistent with our standard basal media offerings.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.